We narrowed to 13,037 results for: Mpl
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pET15b-GreBdm-D41N
Plasmid#129691Purposeexpress E. coli GreB E82C/C68S/D41NDepositorInsertGreB E82C C68S D41N (greB E. coli)
UseTagshis6ExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS2_K58R_Keima
Plasmid#127141DepositorInsertRPS2_Keima (RPS2 Human)
UseLentiviralTagsExpressionMutationK58RPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS2_K275R
Plasmid#127131DepositorInsertRPS2 (RPS2 Human)
UseLentiviralTagsExpressionMutationK275RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_K227/230R_Keima
Plasmid#127146DepositorInsertRPS3_Keima (RPS3 Human)
UseLentiviralTagsExpressionMutationK227R/K230RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS3_K227R_Keima
Plasmid#127144DepositorInsertRPS3_Keima (RPS3 Human)
UseLentiviralTagsExpressionMutationK227RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
A3H HapII R175/6E-Cas9n-UGI
Plasmid#119142PurposeBase editor made from APOBEC3H Haplotype II RNA binding mutant RR175/6EEDepositorInsertAPOBEC3H Haplotype II RR175/6EE-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationA3H Hap II RR175/6EEPromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-Cdc45 (delta 154–164)
Plasmid#126879PurposeExpresses crystallisation construct of human Cdc45 in bacteriaDepositorInsertHuman Cdc45 (CDC45 Human)
UseTagsNo tagExpressionBacterialMutationdeletion amino acid 154-164PromoterT7 promoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti4 Blast RFP-PNRC1
Plasmid#123297PurposeMammalian expression of PNRC1 N-RFPDepositorInsertPNRC1 (PNRC1 Human)
UseLentiviralTagsTurboRFPExpressionMammalianMutationPromoterAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51 PNRC1
Plasmid#123293PurposeExpression of PNRC1 in mammalian cellsDepositorInsertPNRC1 (PNRC1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (I325D)
Plasmid#121412PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationIsoleucine 325 to Aspartic acidPromoterCUP1 promoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (F317D)
Plasmid#121411PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterCUP1 promoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (L105D)
Plasmid#121410PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416)
Plasmid#121408PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17(L313D I314K)
Plasmid#121390PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationLeucine 313 to Aspartic acid; Isoleucine 314 to L…PromoterADH1 promoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (L105D)
Plasmid#121405PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationLeucine 105 to Aspartic acidPromoterCUP1 promoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (F317D)
Plasmid#121406PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationPhenylalanine 317 to Aspartic acidPromoterCUP1 promoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-Atg17(416) (I325D)
Plasmid#121407PurposeExpressing Atg17DepositorInsertCUP1p-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationIsoleucine 325 to Aspartic acidPromoterCUP1 promoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)
Plasmid#119692PurposeFor mammalian expression of LIR double mutant NBR1 without its UBDDepositorInsertNBR1 (NBR1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationF562A/Y731A & UBD deletion (1-906 ), See depo…PromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN iRFP670-OPTN(F178A/D474N)
Plasmid#119694PurposeFor mammalian expression of iRFP-tagged LIR/UBD double mutant OPTNDepositorInsertOPTN (OPTN Human)
UseRetroviralTagsiRFP670ExpressionMammalianMutationF178A/D474NPromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEBAC1-CoREST-C
Plasmid#109159PurposeEncodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)
Plasmid#115432PurposeVector for expressing RPL28 with mutagenized exon2DepositorInsertsUseTagsExpressionYeastMutationexon 2 deleted, with MfeI / NheI cloning sitePromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pUC19_4xT790_28bp
Plasmid#114720Purpose4 x 28 bp repeats of the EGFR WT allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_T790_28bp
Plasmid#114722Purpose28 bp fragment of the WT EGFR allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_19kDa_EE
Plasmid#74951PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsGlu-Glu tag (EYMPME)ExpressionMammalianMutationdelta 1-127PromoterCMV, lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_23kDa_Myc
Plasmid#74949PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsHA Tag (YPYDVPDYA)ExpressionMammalianMutationdelta 1-99 M128APromoterCMV, Lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3- myc NES-mCE(K294A) NLS
Plasmid#82467PurposeExpresses cytoplasmic restricted form of myc tagged mRNA capping enzymeDepositorInsertNLS deleted from mouse Capping Enzyme (Rngtt Mouse)
UseTagsExpressionMammalianMutationdeletion of KRKY from 573-657PromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-biotin-myc- mCE
Plasmid#82475PurposeExpresses mRNA capping enzyme containing N terminal biotin and myc tagDepositorInsertBiotin (Rngtt Mouse)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(WT)
Plasmid#112720PurposeWild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.DepositorInsert6His-TEV-TBP6.7(WT)
UseTags6His-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
UseTags6His-TEVExpressionBacterialMutationP46APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
UseTags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
UseTags6His-TEVExpressionBacterialMutationP51APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
UseTags6His-TEVExpressionBacterialMutationQ54APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Sesn-C mSesn2 (delAB)
Plasmid#111799PurposeMammalian Expression of mSesn2DepositorInsertmSesn2 (Sesn2 Mouse)
UseTagsFlagExpressionMammalianMutationAB domain truncationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Sesn-B+C mSesn2 (delA)
Plasmid#111798PurposeMammalian Expression of mSesn2DepositorInsertmSesn2 (Sesn2 Mouse)
UseTagsFlagExpressionMammalianMutationA domain truncationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Sesn-A mSesn2 (delBC)
Plasmid#111797PurposeMammalian Expression of mSesn2DepositorInsertmSesn2 (Sesn2 Mouse)
UseTagsFlagExpressionMammalianMutationBC domain truncationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_Strep
Plasmid#101651PurposeExpression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_Strep
Plasmid#101652PurposeExpression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMflCT-o4
Plasmid#101313PurposeContains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.DepositorInsertsoriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
cat resistance gene
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 N181-211 (#595)
Plasmid#99939Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationdelAA181-211insNAAIRS linkerPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 N187-207 (#596)
Plasmid#99940Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationdelAA187-207insNAAIRS linkerPromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 85-232 (#427)
Plasmid#99922Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationN-terminal deletion; encodes AA 85-232PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 101-232 (#183)
Plasmid#99923Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationN-terminal deletion; encodes AA 101-232PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 1-192 (#421)
Plasmid#99925Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationC-terminal deletion; encodes AA 1-192PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 1-209 (#430)
Plasmid#99926Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationC-terminal deletion; encodes AA 1-209PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 96-110 (#189)
Plasmid#99929Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationInternal deletion of AA 96-110PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-REDD1 111-135 (#190)
Plasmid#99930Purposemammalian expressionDepositorInsertREDD1 (DDIT4 Human)
UseTagsHAExpressionMammalianMutationInternal deletion of AA 111-135PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only
