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Addgene

We narrowed to 3,069 results for: FRI

Showing: 2561 - 2580 of 3069 results
  1. pHybr_r2a>Fab-srt-his

    Plasmid
    #124810
    Purpose
    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.
    Insert
    rat IgG2a Hinge-G4S-Sortag-Histag
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial
    Mutation
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. hNTa1-qgRNA-pYJA5

    Plasmid
    #217779
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. hNTo1-qgRNA-pYJA5

    Plasmid
    #217781
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. hNTo2-qgRNA-pYJA5

    Plasmid
    #217782
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. hNTa2-qgRNA-pYJA5

    Plasmid
    #217780
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pLenti-EML4-ALK variant 3a G1202R

    Plasmid
    #183830
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells
    Depositor
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    G1202R
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid
    #183831
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells
    Depositor
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    L1196M/G1202R
    Promoter
    CMV
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pQE30-rSyntenin1

    Plasmid
    #89428
    Purpose
    bacterial expression of rat Syntenin1
    Depositor
    Insert
    SDCBP
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    May 3, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. VfAU1-LOV_136

    Plasmid
    #63632
    Purpose
    VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a restriction site
    Depositor
    Insert
    VfAU1-LOV
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. XE141 sFRP-1-CS2+

    Plasmid
    #16693
    Depositor
    Insert
    sFRP-1 (Sfrp1 Mouse)
    Use
    Xenopus expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 22, 2009
    Availability
    Academic Institutions and Nonprofits only
  11. MYR-VfAU1-LOV_135

    Plasmid
    #63631
    Purpose
    Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a MYR signal and a restriction site
    Depositor
    Insert
    MYR-VfAU1-LOV
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. P75ECD-VfAU1-LOV_448

    Plasmid
    #63633
    Purpose
    Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by the extracellular and transmembrane domain of p75NTR and a restriction site
    Depositor
    Insert
    P75ECD-VfAU1-LOV
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  13. mVenus-P75ECD-VfAU1-LOV_458

    Plasmid
    #63634
    Purpose
    Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by mVenus, the extracellular and transmembrane domain of p75NTR and a restriction site
    Depositor
    Insert
    mVenus-P75ECD-VfAU1-LOV
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 2561 - 2580 of 3069 results