We narrowed to 9,521 results for: try

1. pME minprom_creERT2

   Plasmid

   #195966

   Purpose

   Gateway pME CreERT2 ORF

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mouse beta-globin minimal promoter creERT2
   

   Use

   Cre/Lox

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-GST-2

   Plasmid

   #228984

   Purpose

   For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-2
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-GST-1

   Plasmid

   #228983

   Purpose

   For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-1
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMVHA E2F2

   Plasmid

   #24226

   Purpose

   Mammalian expression of human E2F2 with HA tag

   Depositor

   Kristian Helin

   Article

   Lukas et al Mol Cell Biol. 1996 Mar . 16(3):1047-57.

   Insert

   E2F2 (E2F2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFloat.T7_v1

   Plasmid

   #133070

   Purpose

   Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

   Depositor

   David Sabatini, Kacper Rogala

   Article

   Rogala et al Science. 2019 Oct 25;366(6464):468-475.

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   3C-His6 and His6-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. 612 p3E-2A-mCherryCAAXpA

   Plasmid

   #195971

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Christian Mosimann, Kristen Kwan

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-mCherry-CAAX (membrane)
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMuLE (L1-L4) 4X PRE

   Plasmid

   #206167

   Purpose

   pMuLE L1-L4 entry plasmid containing 4 consensus PGR bindings sites upstream of a minimal 2YBTATA promoter

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   4X PRE
   

   Use

   Gateway cloning entry clone

   Promoter

   2YBTATA

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221_HUWE1_isoform1

   Plasmid

   #190464

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. prDNA spacer-LacOx50-20xMS2-Hyg

   Plasmid

   #210219

   Purpose

   Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   50xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22b_PstS_2

    Plasmid

    #78199

    Purpose

    Expression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A17C,A197C)
    

    Expression

    Bacterial

    Mutation

    A17C; A197C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-FAS-GCaMP6f-WPRE

    Plasmid

    #216277

    Purpose

    AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Promoter

    human synapsin 1 (hSyn)

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRetroX-Tight-MCS_PGK-GpNLuc

    Plasmid

    #70185

    Purpose

    Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pR-mKate2

    Plasmid

    #228610

    Purpose

    Mammalian expression of mKate2, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mKate2
    

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroX-Tight-MCS_PGK-OgNLuc

    Plasmid

    #70186

    Purpose

    Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3E-2xFYVE_hrs

    Plasmid

    #67676

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    2xFYVEhrs
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-lox-GFP shRNA p19-2

    Plasmid

    #14091

    Depositor

    Tyler Jacks

    Article

    Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

    Insert

    p19 ARF shRNA -2 (Cdkn2a Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pENTR223-EBNA1

    Plasmid

    #195827

    Purpose

    Gateway Entry Clone of Epstein-Barr virus EBNA1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    EBNA1 (EBNA-1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-KSENB-Nup133

    Plasmid

    #87340

    Purpose

    To express human Nup133 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion of Nup133 AA1-7

    Promoter

    CMV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. 766 p3E-2A-nlsmCherrypA

    Plasmid

    #195972

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-nlsmCherry (nuclear)
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSNRW-Z5-Nluc

    Plasmid

    #174487

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z5-Nluc
    

    Tags

    6x Histidine tag (N terminal on insert)

    Expression

    Bacterial

    Promoter

    T7lac promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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