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Plasmid#112333PurposeCRISPR donor plasmid to tag human transcription factor THRB with GFPDepositorInsertTHRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-KQ
Plasmid#64982PurposeExpresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108QPromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-A
Plasmid#64979PurposeExpresses the E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108APromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-K
Plasmid#64978PurposeExpresses the E105K mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105KPromoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH729-minRLuc
Plasmid#38225DepositorInsertRenilla Luciferase
UseTagsExpressionYeastMutationEvery codon of the Renilla coding sequence was ex…PromoterTDH3 (=GPD)Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH738-CENBEVYslow
Plasmid#38220DepositorInsertGCN4-based single uORF-containing 5'-UTR
UseTagsExpressionYeastMutationThe start codons of uORFs2, 3 and4 of the yeast G…PromoterTDH3 (=GPD)Available sinceDec. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH490-tQSRTL
Plasmid#29699DepositorInserttR(CCG)J; tQ(CUG)M; tT(CGU)K; tS(CGA)C; tL(GAG)G
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH847-gst-optsc
Plasmid#96908PurposePlasmid for yeast expression of GST encoded by preferred codonsDepositorArticleInsertyeast codon optimised glutathione-S transferase
UseTags8xhistidineExpressionYeastMutationPromoterTDH3Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH761-CEN-mCherry_v4
Plasmid#40599DepositorInsertmCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
UseTagsExpressionYeastMutationmCherry ORF partially codon optimised for yeast e…PromoterGPD (TDH3)Available sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTH732-2µ-RLuc/staCFLuc
Plasmid#40607DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3Available sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only