Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 459 results for: SK2

Showing: 361 - 390 of 459 results

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R703-M80-303: CMV51p> Halotag-Kras4bHVR

Plasmid

#159683

Purpose

Mammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Kras4bHVR (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

Plasmid

#156405

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHBT-KRAS174

Plasmid

#248050

Purpose

To express His6 tag-Avi tag-TEV cleavage site-KRAS (residues 1-174) in E. coli, under the T7 promoter.

Depositor

Shohei Koide

Article

Wallon et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

Insert

KRAS (KRAS Human)

Tags

His6 tag-Avi tag-TEV cleavage site

Expression

Bacterial

Mutation

includes aa 1-174 only

Promoter

T7

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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NanoBRET BiBRET Vector, NanoLuc-KRAS(G12V)_CRAF(RBD)-HaloTag

Plasmid

#238572

Purpose

Express NanoLuc-KRAS(G12V)_CRAF(RBD)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
CRAF(RBD) (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, NanoLuc-KRAS(G12D)_CRAF(RBD-CRD)-HaloTag

Plasmid

#238576

Purpose

Express NanoLuc-KRAS(G12D)_CRAF(RBD-CRD)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
CRAF(RBD-CRD) (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, HaloTag-KRAS(G12D)_BRAF-NanoLuc

Plasmid

#238563

Purpose

Express HaloTag-KRAS(G12D)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
BRAF (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, HaloTag-KRAS(G12V)_BRAF-NanoLuc

Plasmid

#238565

Purpose

Express HaloTag-KRAS(G12V)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
BRAF (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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NanoBRET BiBRET Vector, HaloTag-KRAS(G12C)_BRAF-NanoLuc

Plasmid

#238566

Purpose

Express HaloTag-KRAS(G12C)_BRAF-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
BRAF (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12C

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pME-V5-mTagBFP2-KRAS4A-WT (JDW 831)

Plasmid

#242568

Purpose

Gateway middle entry clone containing an mTagBFP2 fused human KRAS4A WT.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A (WT) (KRAS Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-V5-mClover-KRAS4A-WT (JDW 812)

Plasmid

#242566

Purpose

Gateway middle entry clone containing an mClover3 fused human KRAS4A WT.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A (WT) (KRAS Human)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-G12R-IRES-mCherry

Plasmid

#221023

Purpose

Fluorescent reporter for expressing the KRAS G12R mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12R (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-G12S-IRES-mCherry

Plasmid

#221024

Purpose

Fluorescent reporter for expressing the KRAS G12S mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12S (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-WT-IRES-mCherry

Plasmid

#221019

Purpose

Fluorescent reporter for expressing the KRAS wildtype CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS wildtype (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-G12A-IRES-mCherry

Plasmid

#221020

Purpose

Fluorescent reporter for expressing the KRAS G12A mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12A (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-G12C-IRES-mCherry

Plasmid

#221021

Purpose

Fluorescent reporter for expressing the KRAS G12C mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12S (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-KRAS-G12D-IRES-mCherry

Plasmid

#221022

Purpose

Fluorescent reporter for expressing the KRAS G12D mutant CDS

Depositor

Mohamed Fareh

Article

Shembrey et al Sci Adv. 2024 Dec 20;10(51):eadl0731. do

Insert

KRAS G12D (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
NanoBRET BiBRET Vector, NanoLuc-KRAS WT-CRAF RBD-CRD-HaloTag

Plasmid

#236865

Purpose

Express NanoLuc(R)-KRAS WT-CRAF RBD-CRD-HaloTag(R) in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
cRAF(RBD)-CRD (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-KRAS-G12D

Plasmid

#116423

Purpose

Lentiviral expression of KRAS G12D

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12D

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-KRAS

Plasmid

#116755

Purpose

Lentiviral expression of KRAS

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDNA2.0-6H-TEV-KRAS G12D

Plasmid

#159439

Purpose

Expresses KRAS G12D in E.coli

Depositor

Kenneth Westover

Article

Poulin et al Cancer Discov. 2019 Jun;9(6):738-755. do

Insert

KRAS G12D (KRAS Human)

Tags

6xHIS

Expression

Bacterial

Promoter

T5

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12D

Plasmid

#81651

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

G12D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_KRAS_p.G12C

Plasmid

#81667

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

G12C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-V5-mClover-KRAS4A-G12D (JDW 813)

Plasmid

#242567

Purpose

Gateway middle entry clone containing an mClover3 fused human KRAS4A G12D.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

KRAS4A-G12D (KRAS Human)

Use

Gateway subcloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_WT

Plasmid

#81923

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)

Plasmid

#156407

Purpose

pCS2 CMV expression vector driving EGFP fused human KRAS4B-G12V

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4B-G12V (KRAS Human)

Tags

EGFP

Expression

Mammalian

Mutation

mutant KRAS4B-G12V

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_KRAS_p.G12V

Plasmid

#81665

Purpose

Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

G12V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-KRAS-G12V

Plasmid

#116424

Purpose

Lentiviral expression of KRAS G12V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-CSNK2B

Plasmid

#23359

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK2B (CSNK2B Human)

Use

Gateway donor vector

Mutation

C138T

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_KRAS_p.G13D

Plasmid

#82808

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway entry vector

Mutation

G13D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)

Plasmid

#159539

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 361 - 390 of 459 results

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