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pcDNA3-HA-eEF2K(S441A/S445A)

Plasmid

#110161

Purpose

Expression of human eEF2K (S441A/S445A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

Insert

eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)

Tags

HA

Expression

Mammalian

Mutation

S441A/S445A

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCC324

Plasmid

#110165

Purpose

miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

Depositor

Iva Greenwald

Article

de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

Insert

ERK-KTR-mClover-T2A-mCherry-H2B

Use

Minimos transposon

Tags

mCherry and mClover

Expression

Worm

Mutation

R61K change made in ERK-KTR CDS

Promoter

lin-31 promoter/enhancer

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-BE3RA-PGK-Puro

Plasmid

#110846

Purpose

Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

TRE3G

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK301

Plasmid

#110593

Purpose

BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Bba_B0015 Terminator

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-xCas9RA-P2A-Puro

Plasmid

#110871

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)RA

Use

Lentiviral

Tags

FLAG

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-MyosinVb-Ctail

Plasmid

#110170

Purpose

Dominant negative effect by competing with MyosinVb C-terminal tail interactions

Depositor

Jose Esteban

Article

Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

Insert

Myosin Vb (Myo5b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 1262-1670

Promoter

CMV

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMT2-HA-RAPGEF2(S1244A/S1248A)

Plasmid

#110163

Purpose

Expression of human RAPGEF2 (S1244A/S1248A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Tags

HA

Expression

Mammalian

Mutation

S1244A/S1248A

Promoter

CMV

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK402

Plasmid

#110598

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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tet-pLKO.neo_shGFP

Plasmid

#110470

Purpose

Control, silence GFP gene, doxycycline inducible, neomycin selection

Depositor

Sandra Martha Gomes Dias

Article

Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

Insert

GFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1/TO (RNA PolIII)

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-VQR-P2A-GFP-PGK-Puro

Plasmid

#110861

Purpose

Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQR

Use

Lentiviral

Tags

3X FLAG

Mutation

D1135V, R1335Q, T1337R

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-PGK-Puro

Plasmid

#110844

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGL410-BRN2p

Plasmid

#110733

Purpose

Luciferase reporter for the human BRN2 promoter

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRN2 promoter luciferase (POU3F2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PTPN22_Y-phosphatase

Plasmid

#110048

Purpose

Protein expression and purification of PTPN22_Y-phosphatase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PTPN22_Y-phosphatase (PTPN22 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV600

Plasmid

#110723

Purpose

Lentiviral system for reduced ectopic expression.

Depositor

Kevin Dean, Reto Fiolka

Article

Dean et al Biophys J. 2016 Mar 29;110(6):1456-65. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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ARF1 (ARF1A-c001)

Plasmid

#110258

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ARF1 (ARF1 Human)

Tags

StrepII-TEV

Expression

Bacterial

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-Citrine2

Plasmid

#110772

Purpose

Yellow fluorescent protein with improved photostability

Depositor

Robert Campbell

Article

Wiens et al Integr Biol (Camb). 2018 Jul 16;10(7):41

Insert

Citrine2

Tags

His tag

Expression

Bacterial

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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ECFP-Rab5C-S35N

Plasmid

#110504

Purpose

Dominant Negative

Depositor

Terence Dermody, Bernardo Mainou

Article

Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

Insert

Rab5C-S35N (RAB5C Human)

Tags

ECFP

Expression

Mammalian

Mutation

S35N

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBXNPH3

Plasmid

#110098

Purpose

E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-PKCZ

Plasmid

#110512

Purpose

Fluorescent PKCZ

Depositor

Bernardo Mainou

Insert

PKCZ (PRKCZ Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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