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pMpGWB327

Plasmid

#68655

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pQE-WT-T3-R5-RV

Plasmid

#63628

Purpose

T3-R5-RV RNAP mutant

Depositor

Andrew Ellington

Publication

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

T3-R5-RV RNAP

Mutation

T745K, N748D, L749M, M750I, H772R, E775V

Promoter

T5/Lac

Available since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pMX155

Plasmid

#29481

Depositor

Linda Wordeman

Publication

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

Kif2A-alpha (Kif2a Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

E140K

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEE060

Plasmid

#176817

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

611

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PROM5_MpEF1a

Plasmid

#136113

Purpose

Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpEF1a Mapoly0024s0116

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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K-p26

Plasmid

#38783

Depositor

David Wang

Publication

Siebrasse et al J Virol. 2012 Jun 27.

Insert

1334-2611 of MWPyV

Expression

Bacterial

Mutation

One SNP in comparison to MA095

Promoter

lac

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only
pET23a-HsUBB

Plasmid

#69562

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Publication

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBB (UBB Human)

Expression

Bacterial

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFastBac1-Ls-AChBP

Plasmid

#63126

Purpose

Expression of Lymnaea stagnalis AChBP

Depositor

Titia Sixma

Publication

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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L1_CsR-Ck1

Plasmid

#136124

Purpose

L1 in position 1 (next to LB in L2 construct), for CsR

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:mALS_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMpGWB308

Plasmid

#68636

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

MpEF1alpha promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BHMT

Plasmid

#64075

Purpose

Expression of betaine homocysteine methyltransferase

Depositor

Vern Schramm

Publication

Poulin et al J Org Chem. 2015 May 15;80(10):5344-7. d

Insert

betaine-homocysteine methyltransferase (BHMT Synthetic, Human)

Tags

none

Expression

Bacterial

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac-M13-6His-KIF1C-GFP

Plasmid

#130975

Purpose

Expression of 6His-KIF1C-GFP in insect cells for protein purification.

Depositor

Anne Straube

Publication

Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

Insert

KIF1C-GFP (KIF1C Human)

Tags

6His and GFP

Expression

Insect

Mutation

5 silent mutations that make this construct RNAi …

Promoter

Polyhedrin

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIA1363

Plasmid

#166861

Purpose

Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

Depositor

Irina Artsimovitch

Publication

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp8 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCRW0.8 p21 (rat)

Plasmid

#16477

Depositor

Bert Vogelstein

Publication

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 (Cdkn1a Rat)

Expression

Bacterial

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
AL13Rb-35S

Plasmid

#161008

Purpose

PP7-tagged reporter driven by the 35S promoter

Depositor

Krishna Niyogi

Publication

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pGV3319

Plasmid

#170277

Purpose

Expression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)

Depositor

Gregory Van Duyne

Publication

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

Bst-LF D720A

Tags

Hexahistidine

Expression

Bacterial

Mutation

D720A

Promoter

T7-lac

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPM1003

Plasmid

#167550

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

Depositor

Renato Morona

Publication

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Ogawa serotype

Use

Cosmid

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGWB205

Plasmid

#68596

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB228

Plasmid

#68619

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Publication

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO PCGF2

Plasmid

#69791

Purpose

Gateway entry vector for PCGF2

Depositor

Gerard Cagney

Insert

PCGF2 (PCGF2 Human)

Expression

Bacterial

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only