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pAAVCam1.3_smFP_Myc

Plasmid

#201205

Purpose

AAV vector for camKII promoter (1.3kb)-driven smFP_Myc (Myc-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _Myc (from addgene #59757)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

mouse CamKII (1.3kb)

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVCam1.3_smFP_HA

Plasmid

#201206

Purpose

AAV vector for camKII promoter (1.3kb)-driven smFP_HA (HA-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _HA (from addgene #59759)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

mouse CamKII (1.3kb)

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVCam1.3_smFP_FLAG

Plasmid

#201207

Purpose

AAV vector for camKII promoter (1.3kb)-driven smFP_FLAG (FLAG-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _FLAG (from addgene #59756)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

mouse CamKII (1.3kb)

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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AAVTRE3_smFP_FLAG

Plasmid

#201210

Purpose

AAV vector for TRE(TRE3G)-driven smFP_FLAG (FLAG-tagged spaghetti monster) expression

Depositor

Tetsuo Yamamori

Article

Watakabe et al J Vis Exp. 2025 Jan 17;(215). doi: 10.37

Insert

smFP _FLAG (from addgene #59756)

Use

AAV

Expression

Bacterial and Mammalian

Promoter

TRE3G

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pWR95(URA3)

Plasmid

#203917

Purpose

BLINCAR plasmid for gene deletion, URA3 target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

URA3 upstream homology
URA3 downstream homology

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR91

Plasmid

#203915

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at CRF1 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

CRF1 plus flanking (CRF1 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR90

Plasmid

#203914

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

GSC2 plus flanking (GSC2 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR89

Plasmid

#203423

Purpose

empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

EGC3 plus flanking (EGC3 S. stipitis (yeast))

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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BcC _pET29b

Plasmid

#215403

Purpose

Expression construct for BcC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

BcC

Expression

Bacterial

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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OoH_pET29b

Plasmid

#215424

Purpose

Expression construct for OoH gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

OoH

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Ph-NylC_pET28a

Plasmid

#215425

Purpose

Expression construct for Ph-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Ph-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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ThcCut2 _pET29b

Plasmid

#215430

Purpose

Expression construct for ThcCut2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

ThcCut2

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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TtA_pET29b

Plasmid

#215432

Purpose

Expression construct for TtA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

TtA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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MsmegA _pET29b

Plasmid

#215411

Purpose

Expression construct for MsmegA gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

MsmegA

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Nc-NylC_pET28a

Plasmid

#215412

Purpose

Expression construct for Nc-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

Nc-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AoC_pET29b

Plasmid

#215402

Purpose

Expression construct for AoC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

AoC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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TR knockout HRD

Plasmid

#207579

Purpose

Homologous recombination donor replacing TR with a puro cassette.

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

puro resistance cassette flanked by TR locus sequences excluding TR

Tags

None

Expression

Mammalian

Promoter

SV40

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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C-SWAT GFP CL1 DAmP

Plasmid

#182513

Purpose

C-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CL1 and DAmP

Tags

GFP

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.006

Plasmid

#184970

Purpose

Express -Eco1 RT in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Integrating inducible cassette: Eco1 RT

Tags

SV40NLS

Expression

Yeast

Mutation

human codon optimized RT

Promoter

Gal1-10

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.005

Plasmid

#184971

Purpose

Express Cas9 in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Integrating inducible cassette: Cas9

Tags

SV40NLS

Expression

Yeast

Promoter

Gal1-10

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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