We narrowed to 24,400 results for: lars;

1. pmEGFP-tHe-Tub1A1

   Plasmid

   #201728

   Purpose

   mEGFP-tagged tardigrade (Hypsibius exemplaris) alpha-tubulin Tub1A1 for expression in mammalian cells

   Depositor

   Stanislav Vinopal

   Article

   Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

   Insert

   tHe-Tub1A1
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-BLZinCh-P40

   Plasmid

   #188057

   Purpose

   Expression of BLZinCh-P40, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

   Depositor

   Maarten Merkx

   Article

   Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

   Insert

   BLZinCh-P40
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-BLZinCh-P50

   Plasmid

   #188058

   Purpose

   Expression of BLZinCh-P50, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

   Depositor

   Maarten Merkx

   Article

   Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

   Insert

   BLZinCh-P50
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-BLZinCh-P20

   Plasmid

   #188055

   Purpose

   Expression of BLZinCh-P20, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

   Depositor

   Maarten Merkx

   Article

   Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

   Insert

   BLZinCh-P20
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-BLZinCh-P30

   Plasmid

   #188056

   Purpose

   Expression of BLZinCh-P30, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cells

   Depositor

   Maarten Merkx

   Article

   Michielsen et al ACS Chem Biol. 2022 Jun 17;17(6):1567-15

   Insert

   BLZinCh-P30
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promotor

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Rp51a_10bp 5'SS_No BP or pBP_pUC18

   Plasmid

   #100989

   Purpose

   Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Rp51a_SUS1 5'ss_No_BP_pBP-pUC18

   Plasmid

   #100990

   Purpose

   Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…

   Promoter

   Available since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAV-U6+27-Tornado-Squash-SAM-sensor 4-2

   Plasmid

   #177916

   Purpose

   Expresses Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

   Depositor

   Samie Jaffrey

   Article

   Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

   Insert

   Tornado-Squash-SAM-sensor 4-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6+27

   Available since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. tcTRP24sub8_Cap

   Plasmid

   #175797

   Purpose

   de novo thick circular tandem repeat protein capped to prevent multimerization

   Depositor

   Barry Stoddard

   Article

   Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

   Insert

   tcTRP24sub8_Cap
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. tcTRP9

    Plasmid

    #175793

    Purpose

    de novo thick circular tandem repeat proteins with 9 repeats

    Depositor

    Barry Stoddard

    Article

    Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

    Insert

    tcTRP9
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBAD-deLACCO

    Plasmid

    #167945

    Purpose

    Fluorescent biosensor control for extracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    deLACCO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    April 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRII-TOPO CMV-cGFP-SV40 pA + Laccase2 Exon 2

    Plasmid

    #91802

    Purpose

    Expresses the Laccase2 circular RNA when the upstream SV40 poly(A) signal fails to be used

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Methods. 2021 Apr 18. pii: S1046-2023(21

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MtMscL delta49

    Plasmid

    #118342

    Purpose

    M. tuberculosis MscL (delta49) in pET19b

    Depositor

    Douglas Rees

    Article

    Herrera et al Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.

    Insert

    MscL (mscL Mycobacterium tuberculosis)
    

    Use

    Tags

    6x His Tag and Thrombin Cleavage site

    Expression

    Bacterial

    Mutation

    Changed Glutamic Acid (Glu) 102 to Stop Codon

    Promoter

    T7 Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2K-CAGGS-U6-sgRNA-M7-IRES-CFP

    Plasmid

    #114730

    Purpose

    sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M7
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-PRMT9 deltaTPR mutant

    Plasmid

    #67614

    Purpose

    bacterial expression of GST-PRMT9 missing residues 21-139

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    truncation mutant missing aa 21-139

    Promoter

    tac

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

16. GST-PRMT9 deltaN (aa 21-350)

    Plasmid

    #67611

    Purpose

    bacterial expression of GST-PRMT9 missing residues 21-350

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    truncation mutant missing aa 21-350

    Promoter

    tac

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-847

    Plasmid

    #69902

    Purpose

    Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Hy_pMT Laccase2 Sense

    Plasmid

    #69883

    Purpose

    Expresses the Drosophila laccase2 exon 2 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV-U6+27-Tornado-F30-Squash-Broccoli

    Plasmid

    #177918

    Purpose

    Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-F30-Squash-Broccoli
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCASB

    Plasmid

    #190175

    Purpose

    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

    Depositor

    Jessica Tyler

    Article

    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

    Insert

    sgRNA cloning site
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Added 5'-agagacc-3' upstream and 5'…

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge