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We narrowed to 9,355 results for: bli

Showing: 5201 - 5240 of 9355 results

  1. hC73W-pEGFP-N1

    Plasmid
    #111537
    Purpose
    Expresses human KCNB1 C73W in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human C73W-Kv2.1 (KCNB1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    C73W
    Promoter
    CMV
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111537-plasmid-map-sequence-id-211625

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  2. hC73M-pEGFP-N1

    Plasmid
    #111535
    Purpose
    Expresses human KCNB1 C73M in mammalian cells for imaging
    Depositor
    Federico Sesti
    Insert
    human C73M-Kv2.1 (KCNB1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    C73M
    Promoter
    CMV
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111535-plasmid-map-sequence-id-211630

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  3. His8:MBP-tev-Leu-eΔK-Flv

    Plasmid
    #83397
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83397-plasmid-map-sequence-id-175072

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  4. His8:MBP-tev-Arg-eΔK-Flv

    Plasmid
    #83396
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83396-plasmid-map-sequence-id-175052

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  5. His8:MBP-tev-Phe-eΔK-Flv

    Plasmid
    #83395
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used - as control - in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Available Since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83395-plasmid-map-sequence-id-175037

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  6. pVP16-tev-PRT1-C195A

    Plasmid
    #83408
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C195A substitution
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83408-plasmid-map-sequence-id-175084

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  7. pVP16-tev-PRT1-C29A

    Plasmid
    #83407
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C29A substitution
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83407-plasmid-map-sequence-id-175083

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  8. pVP16-tev-PRT1-V28A

    Plasmid
    #83406
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 E. coli, Mustard Weed)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    V28A substitution
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83406-plasmid-map-sequence-id-175082

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  9. pCASCADE-gltA1-gltA2-udhA-zwf

    Plasmid
    #87152
    Purpose
    gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-zwf guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87152-plasmid-map-sequence-id-174941

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  10. pCASCADE-gltA1-gltA2-udhA-GapAP1

    Plasmid
    #87151
    Purpose
    gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-GapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87151-plasmid-map-sequence-id-174940

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  11. pCASCADE-fabI-gltA1-gltA2-gapAP1

    Plasmid
    #87147
    Purpose
    fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-gapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87147-plasmid-map-sequence-id-174935

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  12. pJM103: 150bp Hps2 RFP on pCM66T

    Plasmid
    #74744
    Purpose
    pCM66T with shortened pHps2 promoter driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    pHps2, trimmed (extracted from Methylovorus gluco…
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74744-plasmid-map-sequence-id-141459

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  13. pUC57-sgDedd-2

    Plasmid
    #74436
    Purpose
    sgRNA expression vector for mouse Dedd gene targeting intron 4
    Depositor
    Petra Arck
    Insert
    Dedd (Dedd Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pJK652

    Plasmid
    #72470
    Purpose
    Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)
    Depositor
    T. J. Kappock
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated histidine-233 to glycine
    Promoter
    T7
    Available Since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72470-plasmid-map-sequence-id-134018

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  15. pJK520

    Plasmid
    #72479
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic gene
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Gene synthesis
    Tags
    His6
    Mutation
    synthetic gene
    Promoter
    n/a and none
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72479-plasmid-map-sequence-id-132532

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  16. pJK559

    Plasmid
    #71603
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71603-plasmid-map-sequence-id-131677

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  17. pJK562

    Plasmid
    #71604
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Leu/Trp165>Phe mutant (AaPurE1fwf-Y154L)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71604-plasmid-map-sequence-id-131680

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  18. pJK604

    Plasmid
    #71610
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe mutant (AaPurE1fww-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71610-plasmid-map-sequence-id-131686

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  19. pJK664

    Plasmid
    #71613
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1wwf-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tyrosine-154 …
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71613-plasmid-map-sequence-id-131691

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  20. pJK666

    Plasmid
    #71615
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant (AaPurE1wyf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine, tryptophan-97…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71615-plasmid-map-sequence-id-131694

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  21. pJK563

    Plasmid
    #71689
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Tyr154>Ile/Trp165>Phe mutant (AaPurE1fwf-Y154I)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, tyrosine-…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71689-plasmid-map-sequence-id-131681

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  22. pJK508

    Plasmid
    #71598
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Trp165>Phe mutant (AaPurE1fwf-H59N)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71598-plasmid-map-sequence-id-131672

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  23. pPyCAG-Nanog(Q124A)-IP

    Plasmid
    #62993
    Purpose
    Expresses Mouse Nanog Q124A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q124A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Glutamine 124 (125 AA in Mouse Protein) t…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62993-plasmid-map-sequence-id-107891

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  24. pPyCAG-Nanog(M125A)-IP

    Plasmid
    #62994
    Purpose
    Expresses Mouse Nanog M125A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog M125A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Methionine 125 (126 AA in Mouse Protein) …
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62994-plasmid-map-sequence-id-107893

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  25. pPyCAG-Nanog(Q138A)-IP

    Plasmid
    #62997
    Purpose
    Expresses Mouse Nanog Q138A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q138A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Glutamine 138 (139 AA in Mouse Protein) t…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62997-plasmid-map-sequence-id-104599

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  26. pPyCAG-Nanog(T141A)-IP

    Plasmid
    #62999
    Purpose
    Expresses Mouse Nanog T141A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog T141A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Threonine 141 (142 AA in Mouse Protein) t…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62999-plasmid-map-sequence-id-104602

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  27. pPyCAG-Nanog(Q144A)-IP

    Plasmid
    #63000
    Purpose
    Expresses Mouse Nanog Q144A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Q144A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Glutamine 144 (145 AA in Mouse Protein) t…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63000-plasmid-map-sequence-id-104604

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  28. pPyCAG-Nanog(N145A)-IP

    Plasmid
    #63001
    Purpose
    Expresses Mouse Nanog N145A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog N145A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Asparagine 145 (146 AA in Mouse Protein) …
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63001-plasmid-map-sequence-id-104605

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  29. pPyCAG-Nanog(R147A)-IP

    Plasmid
    #63002
    Purpose
    Expresses Mouse Nanog R147A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog R147A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Arginine 147 (148 AA in Mouse Protein) to…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63002-plasmid-map-sequence-id-104608

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  30. pPyCAG-Nanog(K151A)-IP

    Plasmid
    #63004
    Purpose
    Expresses Mouse Nanog K151A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K151A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Lysine 151 (152 AA in Mouse Protein) to A…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    63004-plasmid-map-sequence-id-104611

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  31. pPyCAG-Nanog(T100A)-IP

    Plasmid
    #62989
    Purpose
    Expresses Mouse Nanog T100A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog T100A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Threonine 100 (101AA) to Alanine
    Promoter
    CAG
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62989-plasmid-map-sequence-id-104167

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  32. pPyCAG-Nanog(F102A)-IP

    Plasmid
    #62990
    Purpose
    Expresses Mouse Nanog F102A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog F102A Mutant (Nanog Mouse)
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 102 (103AA in Mouse Protein…
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62990-plasmid-map-sequence-id-107872

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  33. pLV-Enh eGFP Reporter-muKC2-IGR4

    Plasmid
    #59273
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Intergenic 3kb downstream Krt82
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. pLV-Enh eGFP Reporter-muKC2-IGR7

    Plasmid
    #59276
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    intergenic 4kb upstream krt1
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  35. pLV-Enh eGFP Reporter-muKC2-IGR8

    Plasmid
    #59277
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Intergenic 2kb downstream krt79
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. pLV-Enh eGFP Reporter-muKC2-IGR1

    Plasmid
    #59270
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Intergenic 20kb downstream krt80
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pLV-Enh eGFP Reporter-muEDC-IGR15

    Plasmid
    #59283
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the mouse Epidermal Differentiation Complex.
    Depositor
    Benjamin Yu
    Insert
    mLor-IGR2-3_nn79710
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. pLV-Enh eGFP Reporter-muKC2-IGR2

    Plasmid
    #59271
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    Intergenic 10kb upstream krt80
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. pLV-Enh eGFP Reporter-huKC1-IGR19

    Plasmid
    #59285
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the human keratin type I cluster.
    Depositor
    Benjamin Yu
    Insert
    9kb upstream hKrt14-1700
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pLV-Enh eGFP Reporter-muKC2-IGR6

    Plasmid
    #59275
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intergenic region of the keratin type II cluster.
    Depositor
    Benjamin Yu
    Insert
    intergenic 11kb upstream krt2
    Use
    Lentiviral
    Promoter
    Mu Hsp68 minimal promoter
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5201 - 5240 of 9355 results