We narrowed to 10,973 results for: CHL

1. pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217513

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDC123SB-AtTAS1c-B/c

   Plasmid

   #51772

   Purpose

   Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtTAS1c-B/c
   

   Use

   Plant expression

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5RS-HERCD533

   Plasmid

   #65224

   Purpose

   Expression of dominant negative EGFR in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Redemann et al Mol Cell Biol. 1992 Feb;12(2):491-8.

   Insert

   HERCD533 (EGFR Human)
   

   Expression

   Mammalian

   Mutation

   deleted AA 654-1186

   Promoter

   CMV

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pTC-ApoE-Tet-YAP

   Plasmid

   #85580

   Purpose

   Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (ApoE.HCR.hAAT promoter) in mouse liver

   Depositor

   Ursula Ehmer

   Article

   Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

   Inserts

   CreER
   YAP
   

   Use

   Cre/Lox

   Tags

   Flag (2x)

   Expression

   Mammalian

   Promoter

   ApoE.HCR.hAAT and TRE

   Available Since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEIAV-DIO-TLoop-CHR2YFP

   Plasmid

   #66798

   Purpose

   expression of channelrhodopsin-2-YFP, a light-gated cation channel that can depolarize cells via sodium influx when blue light changes its conformation, using a DIO-based TLoop EIAV vector

   Depositor

   Edward Callaway

   Article

   Cetin et al J Neurophysiol. 2014 May;111(10):2150-9.

   Insert

   Floxed Chr2-YFP
   

   Use

   Lentiviral

   Tags

   citrine

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CMVTetO7 Promoter

   Available Since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA

   Plasmid

   #176743

   Purpose

   Expresses Connexin-43 fused with BioID2 at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Cx43-BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-AtTAS1c-B/c

   Plasmid

   #51774

   Purpose

   Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

   Depositor

   James Carrington

   Article

   Carbonell et al Plant Physiol. 2014 Mar 19.

   Insert

   AtTAS1c-B/c
   

   Use

   Gateway-compatible entry clone

   Mutation

   A. thaliana TAS1c precursor sequence including a …

   Available Since

   April 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUB-nHyPer2 (NLS:HyPer2:NLS)

   Plasmid

   #84730

   Purpose

   Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   HyPer2 targeted to nucleus
   

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBSFI-myr-akt-K179M

   Plasmid

   #49188

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Mutation

   K179M

   Promoter

   T7

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV U6-sgRNA UbC-GFP

    Plasmid

    #106948

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

    Plasmid

    #72888

    Purpose

    fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Insert

    Gaussia luciferase, Channelrhodopsin-2, EYFP
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBZcsx27

    Plasmid

    #89900

    Purpose

    Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx27
    

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUB-nSypHer (NLS:sypHer:NLS)

    Plasmid

    #84731

    Purpose

    Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    nSypHer
    

    Tags

    NLS (nuclear localisation signal and NLS (nuclear…

    Expression

    Plant

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWPXL-Hes1

    Plasmid

    #36983

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Hes1 (Hes1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

15. rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

    Plasmid

    #32483

    Purpose

    Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    ChR2HA-2a-PSAML141F,Y115F:GlyR
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    ChR2 2A

    Promoter

    CAG

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBSFI-myr-akt-S473A

    Plasmid

    #49190

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Mutation

    S473A

    Promoter

    T7

    Available Since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pLV sgRNA-Notch1 #1 GFP

    Plasmid

    #106950

    Purpose

    Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    gRNA targeting Notch1 (Notch1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pY005 (pcDNA3.1-hLb3Cpf1)

    Plasmid

    #69977

    Purpose

    Expresses humanized Lb3Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb3Cpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET28a-MSP1D1deltaH4/2H5

    Plasmid

    #71715

    Purpose

    Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV hSyn1 ChR2 ET-TC 2A tDimer

    Plasmid

    #101361

    Purpose

    High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Inserts

    Channelrhodopsin-2
    synaptophysin-red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E123T , T159C

    Promoter

    human Synapsin

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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