We narrowed to 5,443 results for: SPI

1. pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)

   Plasmid

   #160540

   Purpose

   Expresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

   Inserts

   SARS-CoV-2 NSP7 (ORF1ab Synthetic)
   SARS-CoV-2 NSP8 (ORF1ab Synthetic)
   SARS-CoV-2 NSP12 (ORF1ab Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. SARS-CoV2 Nucleocapsid

   Plasmid

   #153201

   Purpose

   Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

   Depositor

   Peter Klein

   Article

   Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
   

   Use

   Tags

   Myc tag (5X)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pTwist-SARS-CoV-2 Δ18 D614G

   Plasmid

   #164437

   Purpose

   Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. plenti-Lb-Cas12a-2xNLS

   Plasmid

   #155046

   Purpose

   Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS, Myc-tag and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Flag-PGC-1alpha1

   Plasmid

   #45501

   Depositor

   Jorge Ruas, Bruce Spiegelman

   Article

   Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

   Insert

   PGC-1alpha Isoform1 (Ppargc1a Mouse)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Q339K relative to NM_008904

   Promoter

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

   Plasmid

   #198569

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA216-CoV2-RBD-beta variant

   Plasmid

   #194598

   Purpose

   Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

   Depositor

   Ben Murrell

   Article

   Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

   Insert

   CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Flag-PGC-1alpha2

   Plasmid

   #45502

   Depositor

   Jorge Ruas, Bruce Spiegelman

   Article

   Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

   Insert

   PGC-1alpha Isoform2 (Ppargc1a Mouse)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-IRES-blast 3xFlag-SPNS1(R76A)

   Plasmid

   #198570

   Purpose

   SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed arginine R76 to alanine (R76A)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-IRES-blast GFP-SPNS1(E164K)

    Plasmid

    #198572

    Purpose

    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag-GFP

    Expression

    Mammalian

    Mutation

    changed glutamic acid E164 to lysine (E164K)

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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