We narrowed to 4,186 results for: Gca

1. pROS13_IDH1 #4

   Plasmid

   #166101

   Purpose

   This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

   Depositor

   Matthias Heinemann

   Article

   Chen et al bioRxiv

   Insert

   IDH1 sgRNA #4 (IDH1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-sgCTRL-1

   Plasmid

   #83934

   Purpose

   Lentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   sgCTRL-1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_gRNA_IG-DMR-1

   Plasmid

   #159931

   Purpose

   Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse IG-DMR
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. OA-1045D

   Plasmid

   #125006

   Purpose

   Expresses gRNAs targeting hedgehog and wingless

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   wg gRNA (wg Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   u6:3

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. px335-sgRNA-EF(Cas9n)-Ddx42-R

   Plasmid

   #122344

   Purpose

   Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Ddx42
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. px335-sgRNA-EF(Cas9n)-Trim28-R

   Plasmid

   #122339

   Purpose

   Expresses sgRNA targeting mouse Trim28 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Trim28
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. px335-sgRNA-EF(Cas9n)-Ctcf-L

   Plasmid

   #122319

   Purpose

   Expresses sgRNA targeting mouse Ctcf and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Ctcf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. px335-sgRNA-EF(Cas9n)-Etv5-R

   Plasmid

   #122324

   Purpose

   Expresses sgRNA targeting mouse Etv5 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Etv5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. px335-sgRNA-EF(Cas9n)-Peg3-L

   Plasmid

   #122311

   Purpose

   Expresses sgRNA targeting mouse Peg3 and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Peg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. px335-sgRNA-EF(Cas9n)-Rad21-L

    Plasmid

    #122313

    Purpose

    Expresses sgRNA targeting mouse Rad21 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Rad21
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(Ndufa2-g2)-PGKpuroBFP-W

    Plasmid

    #105031

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ndufa2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ndufa2 (Ndufa2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL436 HEK293site3EQR sgRNA

    Plasmid

    #126445

    Purpose

    To cut the human HEK293 site3 locus for integration.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human HEK293 site3 with NGAG PAM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MCM6 E1.2 gRNA

    Plasmid

    #90762

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MCM6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    MCM6 (Guide Designation E1.2)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DYNLRB1 H11.3 gRNA

    Plasmid

    #90664

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DYNLRB1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DYNLRB1 (Guide Designation H11.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

    Plasmid

    #121509

    Purpose

    Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah.intron9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. gRNAs[bTub+Tra].1046D

    Plasmid

    #112693

    Purpose

    express two gRNA targeting bTub & Tra under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. gh71

    Plasmid

    #106731

    Purpose

    expression of gRNA targeting B3GNT5

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    B3GNT5 (B3GNT5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. LRG-neo-sgTAF12(mouse)#4.1

    Plasmid

    #105588

    Purpose

    lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    gRNA targeting TAF12 HFD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPN018

    Plasmid

    #91664

    Purpose

    Express sgRNA targeting human SCN2A

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SCN2A (SCN2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN279

    Plasmid

    #91654

    Purpose

    Express sgRNA targeting human PTN

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    PTN (PTN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge