Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 23,118 results for: Sis

Clear keywords
Showing: 2621 - 2640 of 23118 results

  1. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162687-plasmid-map-sequence-id-321457

    Enlarge

  2. pVZ_PcoaT_SQE1_MRN1

    Plasmid
    #103858
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and MRN1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    marneral synthase 1 (MRN1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized2nd amino acid …
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103858-plasmid-map-sequence-id-202616

    Enlarge

  3. pVZ_PcoaT_SQE1_CAS1

    Plasmid
    #103856
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and CAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    cycloartenol synthase 1 (CAS1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    103856-plasmid-map-sequence-id-202299

    Enlarge

  5. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

    Plasmid
    #174516
    Purpose
    Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.
    Depositor
    Jason W Chin
    Article
    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.
    Inserts
    PylS_1R26 - CBZK
    Af_TyrRS - p-I-Phe
    Ma tRNA pylT(8) with CGA anticodon
    A.fulgidus tRNA with CUA anticodon
    Expression
    Bacterial
    Mutation
    CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…
    Promoter
    GlnS and lpp
    Available Since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    174516-plasmid-map-sequence-id-343328

    Enlarge

  6. pT2iU6Apowriter2N(#355)

    Plasmid
    #184106
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis + in vitro trans…
    Tags
    internal tag: 5myc
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi+SP6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184106-plasmid-map-sequence-id-364015

    Enlarge

  7. pLenti-EML4-ALK variant 3a I1171N

    Plasmid
    #183832
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    I1171N
    Promoter
    CMV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183832-plasmid-map-sequence-id-360487

    Enlarge

  8. pLenti-EML4-ALK variant 3a I1171N/D1203N

    Plasmid
    #183833
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK I1171N/D1203N mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Shiba-Ishii et al Nat Cancer. 2022 Jun 20. pii: 10.1038/s4
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    I1171N/D1203N
    Promoter
    CMV
    Available Since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183833-plasmid-map-sequence-id-360485

    Enlarge

  9. pLlacO1-DP6

    Plasmid
    #242138
    Purpose
    AraC compatible Mutagenesis Circuit (drift plasmid)
    Depositor
    Dominik Niopek
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Sept. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    242138-plasmid-map-sequence-id-484679

    Enlarge

  10. PACE_AP_Pos_pBADProm

    Plasmid
    #242139
    Purpose
    pBAD-sd8-GIII Cassette; AP Pos Circuit for AraC phage-assisted evolution
    Depositor
    Dominik Niopek
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242139-plasmid-map-sequence-id-486013

    Enlarge

  11. PACE_AP_GIII_Titrate

    Plasmid
    #242140
    Purpose
    PSP-LacO Cassette; AP Prop Circuit for AraC phage-assisted evolution
    Depositor
    Dominik Niopek
    Insert
    Gene III
    Expression
    Bacterial
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242140-plasmid-map-sequence-id-484675

    Enlarge

  12. PACE_AP_Neg_pBADProm

    Plasmid
    #242141
    Purpose
    pBAD-SD8-GIII Neg; AP Neg Circuit for AraC phage-assisted evolution
    Depositor
    Dominik Niopek
    Insert
    Gene III Negative
    Expression
    Bacterial
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242141-plasmid-map-sequence-id-486018

    Enlarge

  13. pSB-Bleo STAT3

    Plasmid
    #139184
    Purpose
    Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone.
    Depositor
    Neal Devaraj
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    STAT3
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    139184-plasmid-map-sequence-id-309370

    Enlarge

  14. AtFRD3

    Plasmid
    #117086
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    AtFRD3
    Tags
    His
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117086-plasmid-map-sequence-id-224949

    Enlarge

  15. AtAUX1

    Plasmid
    #117087
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    AtAUX1
    Tags
    His
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117087-plasmid-map-sequence-id-224950

    Enlarge

  16. SLAC1-FL

    Plasmid
    #117100
    Purpose
    protein expression in yeast
    Depositor
    Geoffrey Chang
    Insert
    SLAC1-FL
    Tags
    His
    Expression
    Yeast
    Promoter
    AOX
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    117100-plasmid-map-sequence-id-225011

    Enlarge

  17. pLVU/myc

    Plasmid
    #24179
    Depositor
    Lars Ittner
    Insert
    Chloramphenicol resistance gene (CmR) - ccdB gene
    Use
    Lentiviral; Gateway
    Tags
    Myc
    Expression
    Mammalian
    Available Since
    Nov. 29, 2010
    Availability
    Academic Institutions and Nonprofits only

  18. PLJR965_Rv3297

    Plasmid
    #241235
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv3297) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv3297 sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241235-plasmid-map-sequence-id-483383

    Enlarge

  19. PLJR965_Rv2976c

    Plasmid
    #241234
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2976c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv2976c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241234-plasmid-map-sequence-id-483384

    Enlarge

  20. PLJR965_Rv2464c

    Plasmid
    #241232
    Purpose
    CRISPRi gene silencing plasmid with sgRNA (Rv2464c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis
    Depositor
    Kyu Rhee
    Insert
    Rv2464c sgRNA
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    241232-plasmid-map-sequence-id-483381

    Enlarge

Showing: 2621 - 2640 of 23118 results