We narrowed to 5,111 results for: codon optimized

1. lentiCRISPR - C16orf80 sgRNA 2

   Plasmid

   #70650

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C16orf80
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

2. lentiCRISPR - C3orf17 sgRNA 2

   Plasmid

   #70653

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against C3orf17
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pfMDH_Triple

   Plasmid

   #67733

   Purpose

   Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Article

   Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

   Insert

   Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
   

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   Changed histidine 303 to alanine, arginine 373 to…

   Promoter

   T7

   Available Since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pMA3228

   Plasmid

   #46856

   Purpose

   A lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY mutant (K81M) and myc-tagged destabilized FKBP12

   Depositor

   Mikhail Alexeyev, Troy Stevens

   Article

   Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

   Insert

   ExoY K81M
   

   Use

   Lentiviral

   Tags

   FKBP E31G, R71G, K105E and Myc

   Promoter

   TRE-Tight

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR207 SARS-CoV-2 NSP3_nostop

   Plasmid

   #149306

   Purpose

   Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pOPINB-HOIL-1 helix-RBR (233–510)

   Plasmid

   #193859

   Purpose

   Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli

   Depositor

   Bernhard Lechtenberg

   Article

   Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

   Insert

   HOIL-1 (RBCK1 Human)
   

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   Residues 233–510, codon optimised for expression …

   Promoter

   T7

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223 SARS-CoV-2 NSP6_nostop

   Plasmid

   #149309

   Purpose

   Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pDONR223 SARS-CoV-2 ORF3B_nostop

   Plasmid

   #149320

   Purpose

   Gateway-compatible Entry vector, with insert of ORF3B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF3B
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pDONR223 SARS-CoV-2 NSP5_nostop

   Plasmid

   #149308

   Purpose

   Gateway-compatible Entry vector, with insert of NSP5 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pDONR207 SARS-CoV-2 E_nostop

    Plasmid

    #149321

    Purpose

    Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCMV-T7-NLS(SV40)-(Spyo_Cas9)-6xHis-NLS(SV40)

    Plasmid

    #185706

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(Spyo_Cas9) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    Cas9
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR207 SARS-CoV-2 NSP1_nostop

    Plasmid

    #149304

    Purpose

    Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pDONR223 SARS-CoV-2 NSP9_nostop

    Plasmid

    #149312

    Purpose

    Gateway-compatible Entry vector, with insert of NSP9 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pDONR223 SARS-CoV-2 ORF8_nostop

    Plasmid

    #149326

    Purpose

    Gateway-compatible Entry vector, with insert of ORF8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF8 (ORF8 SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pDONR207 SARS-CoV-2 ORF3A_nostop

    Plasmid

    #149319

    Purpose

    Gateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pDONR223 SARS-CoV-2 NSP16_nostop

    Plasmid

    #149318

    Purpose

    Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pDONR207 SARS-CoV-2 M_nostop

    Plasmid

    #149322

    Purpose

    Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    M (M SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pDONR223 SARS-CoV-2 ORF6_nostop

    Plasmid

    #149323

    Purpose

    Gateway-compatible Entry vector, with insert of ORF6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF6 (ORF6 SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDONR207 SARS-CoV-2 ORF7A_nostop

    Plasmid

    #149324

    Purpose

    Gateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    ORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDONR223 SARS-CoV-2 NSP2_nostop

    Plasmid

    #149305

    Purpose

    Gateway-compatible Entry vector, with insert of NSP2 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge