We narrowed to 4,947 results for: mcs

1. pLV-dt/deltaEGFP

   Plasmid

   #163919

   Purpose

   PGK-driven dTomato expression. Lacks the CBA-promoter controlling EGFP expression.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   dtomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-PGK-dt/CBA-EGFP-NLS

   Plasmid

   #163918

   Purpose

   Contains bidirectional reporter genes dtomato and nuclear-targeting EGFP.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   dtomato and EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Added nuclear localization signal to EGFP

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP N1 AURKA deltaNter

   Plasmid

   #157753

   Purpose

   Expression of AURKA without Nter, fused to GFP in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1 AURKA deltaNter

   Plasmid

   #157749

   Purpose

   Expression of AURKA without Nter in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Expression

   Mammalian

   Mutation

   the first 30AA of Aurora A are lacking

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTripZ-B2

   Plasmid

   #163924

   Purpose

   For making a cell line that expresses doxycyline-inducible RNAi suppressor protein B2 for increasing titer of lentivirus with bidirectional promoters during virus production.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   Flock house virus B2 protein (FHVs1gp2 )
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPC-Flag-SOCS1-Y80D

   Plasmid

   #129516

   Purpose

   Retroviral vector for the expression of human SOCS1 with a Y80D mutation and a FLAG tag in N-terminal

   Depositor

   Gerardo Ferbeyre

   Article

   Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

   Insert

   SOCS1 (SOCS1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 80 to Aspartic acid

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPC-Flag-SOCS1-Y80F

   Plasmid

   #129517

   Purpose

   Retroviral vector for the expression of human SOCS1 with a Y80F mutation and a FLAG tag in N-terminal

   Depositor

   Gerardo Ferbeyre

   Article

   Saint-Germain et al Cancer Res. 2019 Jul 1;79(13):3306-3319.

   Insert

   SOCS1 (SOCS1 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Changed Tyrosine 80 to Phenylalanine

   Promoter

   cmv

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Empty Bridge Control Zfp462-Klf4SE

   Plasmid

   #127666

   Purpose

   Encodes for 4 gRNAs expressed from their individual hU6 promoters. Two gRNAs target the Zfp462 promoter while the other two target the Klf4 stretch enhancer.

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Insert

   gRNAs 129, 135, 115, 117
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFL-SV40-TAK1 5'-UTR

   Plasmid

   #115356

   Purpose

   firefly luciferase (Fluc) reporter containing the 5’-UTR of TAK1 mRNA

   Depositor

   Ming-Chih Lai

   Article

   Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

   Insert

   TAK1 5'-UTR (MAP3K7 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-TPI_WT-TNFalpha_3UTR-xrRNA-4H

    Plasmid

    #108374

    Purpose

    Expresses wild type TPI reporter; contains partial TNFalpha 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type TPI reporter with partial TNFalpha control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, TNF Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-HDAC4*

    Plasmid

    #71402

    Purpose

    CAG promoter, mammalian expression

    Depositor

    Bo Chen

    Article

    Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

    Insert

    HDAC4* (HDAC4 Human)
    

    Expression

    Mammalian

    Mutation

    After aa126, insertion leads to reading frame shi…

    Promoter

    CAG

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

12. KHBD00012

    Plasmid

    #34174

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8328 (HLHmdelta Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCDH-CMV-mCherry-EF1 Hygro

    Plasmid

    #129440

    Purpose

    express mCherry

    Depositor

    Oskar Laur

    Insert

    mcherry
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-UBC-LUC-EF1 Hygro

    Plasmid

    #129436

    Purpose

    express LUC

    Depositor

    Oskar Laur

    Insert

    LUC
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDH-EF1s

    Plasmid

    #72484

    Purpose

    Express gene of interest under truncated EF-1 promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCDH-CMV-Nluc

    Plasmid

    #73038

    Purpose

    Express Nluc reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTol2-Synapse reporters

    Plasmid

    #196899

    Purpose

    zebrafish expression

    Depositor

    Reinhard Köster

    Insert

    Synapse reporters
    

    Use

    Zebrafish expression

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAM-PAT-35S-GW-Luc

    Plasmid

    #80678

    Purpose

    binary DEST vector for plant expression

    Depositor

    Nico Dissmeyer, Imre Somssich

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    Pro35S

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-EF1S-Nluc

    Plasmid

    #73033

    Purpose

    Express Nluc

    Depositor

    Kazuhiro Oka

    Insert

    Nluc
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    Truncated EF1

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-EF1S-copGFP

    Plasmid

    #73034

    Purpose

    Express copGFP reporter

    Depositor

    Kazuhiro Oka

    Insert

    copGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Truncated EF1

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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