We narrowed to 10,632 results for: t7

1. pAIP4_1TJ6

   Plasmid

   #234167

   Purpose

   Heterologous protein expression of sprouty-related, EVH1 domain-containing protein 1 (Spred-1) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   1TJ6 (spred1 Xenopus tropicalis)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAIP4_2H3L

   Plasmid

   #234160

   Purpose

   Heterologous protein expression of erbin (Densin-180-like protein) (Erbb2-interacting protein) in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2H3L (ERBIN Human)
   

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCDNA3.1-CLIPf

   Plasmid

   #200787

   Purpose

   For the mammalian expression of CLIPf-HisTag-FLAG fusion protein.

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   CLIPf-HisTag-FLAG
   

   Tags

   CLIPf

   Expression

   Mammalian

   Promoter

   CMV & T7

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1-SNAPf

   Plasmid

   #200788

   Purpose

   For the mammalian expression of SNAPf-HisTag-FLAG fusion protein.

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   SNAPf-HisTag-FLAG
   

   Tags

   SNAPf

   Expression

   Mammalian

   Promoter

   CMV & T7

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6xHis-GFP(-12)

   Plasmid

   #219420

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Insert

   GFP(-12)
   

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD(P+T)_T7

   Plasmid

   #223854

   Purpose

   4G-cloning donor for bacterial promoter and terminator, no tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   T7 promoter and terminator
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PE-SUMO CRYGA

   Plasmid

   #217672

   Purpose

   For expression of SUMO tagged human gamma A crystallin

   Depositor

   Larry David

   Insert

   CRYGA (CRYGA Human)
   

   Tags

   6XHis and Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 8GCY

   Plasmid

   #214440

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CBLB:PBC020-G02:C248501 (CBLB Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 11, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pT7CFE1_EVD68_VP12A_C107R

   Plasmid

   #212982

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A C107R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   changed cysteine 107 from 2A to Arginine

   Promoter

   T7

   Available Since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7CFE1_EVD68_3ABC_C147R

    Plasmid

    #212978

    Purpose

    Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 3ABC C147R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    changed Cysteine 147 in 3C to Arginine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7CFE1_EVD68_3ABCD_C147R

    Plasmid

    #212980

    Purpose

    Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 3ABCD C147R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    changed cysteine 147 from 3C to arginine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7CFE1_EVD68_VP12A_N84T

    Plasmid

    #212983

    Purpose

    Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    EV-D68 MO/14/18947 VP12A N84T
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    changed Asparagine 84 from 2A to Threonine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-Flag-RBM24-1~93

    Plasmid

    #208633

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 1~93, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-Flag-RBM24-95~236

    Plasmid

    #208634

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 95~236, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET24a_thyA_R166Q_untagged

    Plasmid

    #191307

    Purpose

    Bacterial expression of E. coli thyA (thymidylate synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    thyA
    

    Expression

    Bacterial

    Mutation

    R166Q TYMS

    Promoter

    T7

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24a_thyA_Q33S_untagged

    Plasmid

    #191308

    Purpose

    Bacterial expression of E. coli thyA (thymidylate synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    thyA
    

    Expression

    Bacterial

    Mutation

    Q33S TYMS

    Promoter

    T7

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET24a_thyA_R127A_untagged

    Plasmid

    #191309

    Purpose

    Bacterial expression of E. coli thyA (thymidylate synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    thyA
    

    Expression

    Bacterial

    Mutation

    R127A TYMS

    Promoter

    T7

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET24a_thyA_WT_untagged

    Plasmid

    #191306

    Purpose

    Bacterial expression of E. coli thyA (thymidylate synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    thyA
    

    Expression

    Bacterial

    Mutation

    WT TYMS

    Promoter

    T7

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJC20_cam1

    Plasmid

    #182426

    Purpose

    Bacterial expression of the Schizosaccharomyces pombe fission yeast calmodulin, Cam1

    Depositor

    Dan Mulvihill

    Article

    Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

    Insert

    cam1 (cam1 Fission Yeast)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCell-Free_KA1_SARS-COV-2_S-frag3

    Plasmid

    #169403

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    N-term frag. S protein after the furin cleavag (N SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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