We narrowed to 159,134 results for: addgene

1. pGST-HsSyx-375-792

   Plasmid

   #179292

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   His6 + GST + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   residues 375-792 from accession number NP_0010361…

   Promoter

   T7

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUMO-HsSyx3

   Plasmid

   #179288

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + SUMO + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   His6 + SUMO + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

   Plasmid

   #170808

   Purpose

   PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance

   Depositor

   Kenneth Yamada

   Article

   Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

   Inserts

   GID1-VPR-IRES-HygR
   GAI-tagBFP-Sa_dCas9 (GAI Synthetic, Mustard Weed, S. aureus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Synonymous mutation in PYL1 to remove XhoI site (…

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. human ETV6 gRNA-4

   Plasmid

   #133404

   Purpose

   human ETV6 gRNA-3 and 4 is a pair of gRNAs. Four ETV6 gRNA plasmids used the same backbone(Addgene plasmid#41824). ETV6 gRNA-4 target the second ETV6 exon.

   Depositor

   Linzhao Cheng

   Article

   Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

   Insert

   ETV6 (ETV6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCriprV2-sgRNA-CRY2-#1

   Plasmid

   #189989

   Purpose

   Lentiviral Cas9/sgRNA vector targeting C-terminus of hCRY2, works with Addgene 189983-189986

   Depositor

   Achim Kramer

   Article

   Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

   Insert

   Cryptochrome-2 (CRY2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mito-CCM2-mScarlet-I

   Plasmid

   #208886

   Purpose

   Express mScarlet-I-CCM2 targeted to the mitochondria in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM2 (CCM2 Human)
   

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   CCM2 targeted to the mitonchondria

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGFP-HsSyx3

   Plasmid

   #179289

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   His6 + GFP + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHsSyx3-MBP

   Plasmid

   #179286

   Purpose

   E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Insert

   Syx
   

   Use

   Tags

   TEV protease cleavage site + mature maltose bindi…

   Expression

   Bacterial

   Mutation

   residues 393-792 from accession number NP_0010361…

   Promoter

   T7

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGK-Ptrf-mKate2

   Plasmid

   #128765

   Purpose

   Fluorescent mKate reporter for Ptrf

   Depositor

   Deepak Srivastava

   Article

   Stone et al Cell Stem Cell. 2019 Jul 3;25(1):87-102.

   Insert

   Ptrf promoter (Cavin1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ptrf

   Available since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK7

    Plasmid

    #10883

    Depositor

    John Blenis

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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