Addgene

We narrowed to 13,587 results for: nts

Showing: 3961 - 3990 of 13587 results

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LentiCRISPRv2-bleo-PTGER2

Plasmid

#184724

Purpose

EP2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog EP2 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-bleo-PLA2G4A_3

Plasmid

#184725

Purpose

cPLA2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog cPLA2 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-hyg2-PTGER4

Plasmid

#184726

Purpose

EP4-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog EP4 gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS1-3

Plasmid

#184727

Purpose

COX1-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX1 gene.

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS1-4

Plasmid

#184728

Purpose

COX1-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX1 gene.

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-canis_PTGS2-3

Plasmid

#184729

Purpose

COX2-KO in MDCK

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the dog COX2 gene.

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPBpuro-UbC-4493NES(Booster-PKA)

Plasmid

#184711

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIhyg-iRFP670-P2Av3-RGECO1.0

Plasmid

#184713

Purpose

Calcium biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

GCaMP6s

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ300

Plasmid

#205414

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoter

Depositor

Yiping Qi

Article

Li et al Front Genome Ed. 2023 Oct 12;5:1251903.

Insert

Ev1Cas12a

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS (N terminal on insert) NLS (C terminal on ins…

Expression

Plant

Mutation

Promoter

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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G1095C_BattB(GA)_jGCaMP7c_IRES_shBleR-P2A-HygroR

Plasmid

#200638

Purpose

jGCaMP7c recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

jGCaMP7c

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF- RPA4 (MSW#716)

Plasmid

#208077

Purpose

Expression of human Replication Protein A 4 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4 (RPA4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pPath-BGC08-pT7native

Plasmid

#200005

Purpose

BGC08 pathway. pT7-native CDS sequence

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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YP46

Plasmid

#199987

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 46

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-jAspSnFR3-mRuby3

Plasmid

#203459

Purpose

jAspSnFR3-mRuby3 in adenoviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3

Use

AAV and Adenoviral

Tags

His-tag and mRuby3

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pYP-ptef1-GFP

Plasmid

#199990

Purpose

Yeast/E coli shuttle vector expressing GFP from tef1 promoter

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pPath-Violacein-native

Plasmid

#200001

Purpose

Violacein pathway. Native promoter and CDS sequence

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Pathways

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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YP48

Plasmid

#199989

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 48

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YP30

Plasmid

#199971

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 30

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YP16

Plasmid

#199957

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 16

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YP15

Plasmid

#199956

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 15

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YP13

Plasmid

#199954

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 13

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YP7

Plasmid

#199948

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 7

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YP2

Plasmid

#199943

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 2

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YP1

Plasmid

#199942

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 1

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YP28

Plasmid

#199969

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 28

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR1a-jAspSnFR3-mRuby3-NLS

Plasmid

#203489

Purpose

Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Gateway entry vector

Tags

Nuclear localization signal and mRuby3

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR1a-MTS-jAspSnFR3-mRuby3

Plasmid

#203490

Purpose

Mitochondrial directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3

Use

Gateway entry vector

Tags

His-tag, Mitochondrial localization signal, and m…

Expression

Mutation

Promoter

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGWT35S-TP-UnaG

Plasmid

#203484

Purpose

For particle bombardment. A plastid transit peptide fused UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused UnaG

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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YP47

Plasmid

#199988

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 47

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
YP45

Plasmid

#199986

Purpose

Yeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 45

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge