We narrowed to 5,111 results for: codon optimized

1. pDONR223 SARS-CoV-2 ORF7B_nostop

   Plasmid

   #149325

   Purpose

   Gateway-compatible Entry vector, with insert of ORF7B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pDONR223 SARS-CoV-2 NSP10_nostop

   Plasmid

   #149313

   Purpose

   Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pDONR223 SARS-CoV-2 ORF9B_nostop

   Plasmid

   #149327

   Purpose

   Gateway-compatible Entry vector, with insert of ORF9B gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF9B
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pDONR223 SARS-CoV-2 NSP14_nostop

   Plasmid

   #149316

   Purpose

   Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pDONR223 SARS-CoV-2 NSP15_nostop

   Plasmid

   #149317

   Purpose

   Gateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pDONR223 SARS-CoV-2 NSP7_nostop

   Plasmid

   #149310

   Purpose

   Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pDONR223 SARS-CoV-2 NSP4_nostop

   Plasmid

   #149307

   Purpose

   Gateway-compatible Entry vector, with insert of NSP4 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pDONR223 SARS-CoV-2 NSP8_nostop

   Plasmid

   #149311

   Purpose

   Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pDONR223 SARS-CoV-2 ORF9Bwu_nostop

   Plasmid

   #149328

   Purpose

   Gateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF9Bwu
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)

    Plasmid

    #185701

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(FCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)

    Plasmid

    #185705

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(PDCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)

    Plasmid

    #185703

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(SCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)

    Plasmid

    #185704

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(PCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)

    Plasmid

    #185702

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLS

    Depositor

    Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    anCas(BCA)
    

    Tags

    6xHis-NLS and NLS

    Expression

    Mammalian

    Mutation

    optimized for human codon usage

    Promoter

    CMV and T7

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPICZc-HsACTG1*-thymosinB-8His

    Plasmid

    #111147

    Purpose

    Expresses human gamma-actin (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    actin gamma 1 (ACTG1 Human)
    

    Use

    Pichia pastoris integration vector

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    codon-optimised for expression in Pichia pastoris

    Promoter

    AOX1

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid

    #108362

    Purpose

    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

    Depositor

    Luisa Cochella

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Inserts

    Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    

    Tags

    2xFlag, MYC, T2A peptide, and TY1 linker

    Expression

    Worm

    Promoter

    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR221-SARS-CoV-2 S1 RBD

    Plasmid

    #167013

    Purpose

    Gateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2

    Depositor

    Spyros Petrakis

    Article

    Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Expression

    Mammalian

    Mutation

    No stop codon

    Available Since

    April 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. LIC4B-His-TEV-TAK1-TAB1

    Plasmid

    #154874

    Purpose

    Insect cell optimized TAK1-TAB1 construct used to solve structure and deposit PDB ID: 4O91

    Depositor

    Kenneth Westover

    Article

    Tan et al J Med Chem. 2015 Jan 8;58(1):183-96. doi

    Insert

    TAK1-TAB1 (TAB1 Human)
    

    Tags

    LIC TEV His

    Expression

    Insect

    Mutation

    Codon optimized

    Promoter

    polyhedrin

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #42230

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSP1830

    Plasmid

    #70708

    Purpose

    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Insert

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    CAG

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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