We narrowed to 3,663 results for: golden gate

1. L0-I35 p35S-XVE-pEstradiol GG0-GG1

   Plasmid

   #195904

   Purpose

   Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   p35S-XVE-LexA-p35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGM4723:TpCC_Urease

   Plasmid

   #85982

   Purpose

   Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Inserts

   Cas9
   Urease-targeting gRNA 1
   Urease-targeting gRNA 2
   

   Use

   CRISPR; Diatom (t. pseudonana)

   Tags

   NLS and YFP

   Expression

   Mutation

   Promoter

   FCP and U6

   Available since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCB092 FoMV-sgMgCh

   Plasmid

   #234815

   Purpose

   To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbMgCh (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Insert

   gRNA targeting SbMgCh
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCB094 FoMV-sgLw

   Plasmid

   #234816

   Purpose

   To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Insert

   gRNA targeting SbLw
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGY037 BSMV-gamma-AmCyan

   Plasmid

   #234818

   Purpose

   To express flourescent protein AmCyan from the gamma genome of Barley Stripe Mosaic Virus Vector (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Insert

   AmCyan
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICSL90002

   Plasmid

   #68261

   Purpose

   Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter: U6-26 (Arabidopsis thaliana)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC-GoldyTALEN

   Plasmid

   #38143

   Purpose

   destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

   Depositor

   Daniel Carlson

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

   Insert

   GoldyTALEN
   

   Use

   Tags

   AcV5 and FokI homodimer

   Expression

   Mammalian

   Mutation

   Truncate at N and C terminous

   Promoter

   miniCaggs

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. RCIscript-GoldyTALEN

   Plasmid

   #38142

   Purpose

   destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

   Depositor

   Daniel Carlson, Stephen Ekker

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

   Insert

   GoldyTALEN
   

   Use

   Mrna transcription vector

   Tags

   AcV5 and FokI homodimer

   Expression

   Mutation

   Truncate at N and C terminous

   Promoter

   T3

   Available since

   Sept. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRAM28

    Plasmid

    #234314

    Purpose

    pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications

    Depositor

    James Chappell

    Article

    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

    Insert

    U1367 Targeting RAM, Barcode 2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    P. CymRC

    Available since

    March 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIVT-p53DD

    Plasmid

    #232751

    Purpose

    IVT template for making modRNA encoding p53DD, a mutant p53 that lacks DNA binding ability

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    p53DD
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    deletion of DNA binding domain

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNP566

    Plasmid

    #222724

    Purpose

    pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCB036 FoMV-sgGFP

    Plasmid

    #234813

    Purpose

    To express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    gRNA targeting GFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-entry-puro

    Plasmid

    #85745

    Purpose

    Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTBL3478 PB-PEmax-mTagBFP2

    Plasmid

    #226664

    Purpose

    PiggyBac cargo vector encoding PEmax and mTagBFP2

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Inserts

    PEmax
    mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and UBC

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNP1830

    Plasmid

    #222728

    Purpose

    pNP561 - T5 Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pShHELIX_sgRNA_entry (CJT32)

    Plasmid

    #181782

    Purpose

    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIF1079

    Plasmid

    #237445

    Purpose

    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

    Depositor

    IIya Finkelstein

    Article

    Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/

    Inserts

    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG, H1, and U6

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEC-red

    Plasmid

    #196040

    Purpose

    Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

    Depositor

    Paola Branduardi

    Article

    Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Grna cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PDCD1-DEM (P1#5)

    Plasmid

    #227738

    Purpose

    Designer Epigenome Modifier (DEM) designed to target the PDCD1-promoter

    Depositor

    Claudio Mussolino

    Article

    Azcona et al Methods Mol Biol. 2024;2842:209-223. doi

    Insert

    Designer epigenome modifier targeting PDCD1-promoter
    

    Use

    TALEN

    Tags

    DNMT3A, DNMT3L, KRAB

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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