We narrowed to 6,754 results for: hwa

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GALT-SEP-mRuby3

Plasmid

#200938

Purpose

trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

GalT (B4GALT1 Human)
SEP-mRuby3

Use

Lentiviral

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_mCherry sgBFP

Plasmid

#192929

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_mCherry sgAAVS1

Plasmid

#192480

Purpose

All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as control

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A mCherry

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Axin2-d2EGFP

Plasmid

#21276

Depositor

Frank Costantini

Article

Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Insert

Axin2 promoter (Axin2 Mouse)

Tags

d2EGFP

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCarCBADE_ProB(I69E)A

Plasmid

#117756

Purpose

ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Glutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCarCBAE

Plasmid

#117755

Purpose

Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

Depositor

Greg Bokinsky

Article

Shomar et al Nat Chem Biol. 2018 Aug;14(8):794-800. d

Insert

Codon optimised Carbapenem synthase (CarC), Carboxymethylproline synthase (CarB), Carbapenam synthetase (CarA), Ferredoxin CarE

Expression

Bacterial

Promoter

PLac(UV5)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH c-Met

Plasmid

#86498

Purpose

Mammalian expression of human c-Met

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1a

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Hia5-OPT1

Plasmid

#231968

Purpose

Expresses DNA adenine N6-methyltransferase Hia5 in Escherichia coli (E.coli) T7 Express lysY/Iq

Depositor

Schahram Akbarian

Article

Peter et al Cell Rep Methods. 2024 Nov 25:100911. do

Insert

DNA adenine N6-methyltransferase Hia5

Tags

Histidine tag

Expression

Bacterial

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S S85C

Plasmid

#184410

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-c-Met

Plasmid

#31784

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/L55P

Plasmid

#238121

Purpose

bacterial expression of TTR

Depositor

Peter Wright

Article

Sun et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, S85C, L55P

Promoter

T7

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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7xE-Box::Renilla

Plasmid

#124532

Purpose

c-Myc Renilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

c-Myc Renilla reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMK1254

Plasmid

#133057

Purpose

Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.

Depositor

Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

Tau.K18(LM)

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-Uricase

Plasmid

#182417

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-uricase

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCIBN(deltaNLS)-pmGFP

Plasmid

#26867

Purpose

Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

CMV

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse Egr3

Plasmid

#107998

Purpose

Expresses mouse Egr3 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr3 (Egr3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTYMS

Plasmid

#217430

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TYMS

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRi(v1)-Blast

Plasmid

#170067

Purpose

Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

Depositor

Neville Sanjana

Article

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Promoter

EFS-NS

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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VPS13B^GFP

Plasmid

#224588

Purpose

codon optimized GFP tagged VPS13B for expression in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

VPS13B^GFP (VPS13B Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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CBE-T1.52

Plasmid

#193285

Purpose

Expression of CBE-T1.52 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.52

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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