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Plasmid#207894PurposeLevel 0 for C1 module (5' GGCT-3' AGCC)DepositorInsertdummy 20nt sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDiySpike1
Plasmid#136948PurposePlasmid for the In Vitro transcription of DiySpike1DepositorInsertDiySpike1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR540
Plasmid#207885Purposetemplate for 2 gRNA amplificationDepositorInsertscaffold-pU6
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pADH139
Plasmid#90987PurposeNAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.DepositorInsertgRNA conserved, C, mal LEU2 2 of 2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL7Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAT15483_e-ABE8e
Plasmid#174121PurposeExpresses ABE8e fused with eCas9DepositorInserteABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15001-AncBE4max
Plasmid#163016Purposeplasmid expressing AncBE4maxDepositorInsertAncBE4max
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC_CRISPR_Cat
Plasmid#120368PurposeReporter plasmid for full-site selection assayDepositorInsertCRISPR_Cat
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVD_CRISPR
Plasmid#120370PurposePlasmid containing shortened version of V-D CRISPR array for integration assaysDepositorInsertVD_CRISPR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIF251
Plasmid#133935PurposeKanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.DepositorInsertKanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda
UseΛ-phage recombineering plasmidTagsExpressionMutationPromoterAvailable sinceMarch 3, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits