We narrowed to 7,680 results for: CCH
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Plasmid#84531PurposeThis plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
TagsHAExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterADH1Available SinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3NR
Plasmid#84533PurposeThis plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeastDepositorAvailable SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3CR
Plasmid#84534PurposeThis plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeastDepositorAvailable SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K9R
Plasmid#84535PurposeThis plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
TagsEGFPExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterGPDAvailable SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K462-463-651R
Plasmid#84564PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K43-117-118R
Plasmid#84565PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorAvailable SinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGS1212
Plasmid#63785PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection markerDepositorInserteGFP-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseGFP-Strep-tag IIPromoternoneAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB6 Cterminal tail
Plasmid#60403PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable SinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
ER-GFP pLVX CMVpuro
Plasmid#246912PurposeLentiviral-based expression of EGFP with an ER retention signal sequence in mammalian cellsDepositorInsertEGFP
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
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TtEnd5A_pEHISTEV
Plasmid#179732PurposeTtEnd5A_pEHISTEV expression vector encodes first Thermogutta terrifontis Endoglucanase (19-531 amino acid residues) of Glycoside Hydrolase Family 5.DepositorInsertTtEnd5A
Tags6xHisExpressionBacterialMutation19 Amino acids from N-terminal encoding Signal pe…PromoterT7Available SinceOct. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSmBit-CHIP-G132N
Plasmid#236065PurposeSmBit-CHIP expression with G132N substitutionDepositorAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pSmBit-CHIP-K30A
Plasmid#236066PurposeSmBit-CHIP expression with K30A substitutionDepositorAvailable SinceSept. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-ScVip1-186-1107
Plasmid#238316PurposeScVip1-186-1107 protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-186-1107Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
1pBB3-ScVip1-536-1107-C793A
Plasmid#238324Purpose1ScVip1-536-1107-C793A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-C793AAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-186-522
Plasmid#238311PurposeScVip1-186-522 protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-186-522Available SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO979
Plasmid#235756PurposeProtein expression of ScVPS38, untagged + ScVPS30 FRK to DDD at 430-432, untaggedDepositorExpressionYeastMutationFRK430-432DDDAvailable SinceJuly 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-H548A
Plasmid#238319PurposeScVip1-536-1107-H548A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-H548AAvailable SinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107
Plasmid#238312PurposeScVip1-536-1107 protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107Available SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-K554A
Plasmid#238329PurposeScVip1-536-1107-K554A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-K554AAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-R547A
Plasmid#238318PurposeScVip1-536-1107-R547A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-R547AAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-R551A
Plasmid#238320PurposeScVip1-536-1107-R551A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-R551AAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-E991A
Plasmid#238321PurposeScVip1-536-1107-E991A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-E991AAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBB3-ScVip1-536-1107-H651A
Plasmid#238323PurposeScVip1-536-1107-H651A protein expression in insect cellsDepositorInsertVIP1 (VIP1 Budding Yeast)
TagsHis10-Tag, Twin-Strep-tag, TEVExpressionInsectMutationScVip1-536-1107-H651AAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAL007
Plasmid#233866PurposeMoClo-YTK URA multigene plasmid with MCS; NS3 systems; pTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2DepositorInsertpTDH3-LexA-NLS-NS3-V1-VP48-2xOaf1-tADH1; lexAO6-pLEU2m-MCS-tENO2
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO359
Plasmid#235750PurposeProtein expression of FL ScVPS34 and FL ScVPS15-ZZDepositorTags3xTEV-ZZExpressionYeastMutationS2A, aa 3-887 and T134A, I851RPromoterGAL-TDH3Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6-CFAPoptim-KAN
Plasmid#221128PurposePlasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMXDepositorInsertFAPoptim
ExpressionYeastAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC63-FAPoptim
Plasmid#221116PurposeFAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertFAPoptim-SEC63
ExpressionYeastPromoterTEF1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC61-FAPoptim
Plasmid#221115PurposeFAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertSEC61-FAPoptim
ExpressionYeastPromoterTEF1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEW66
Plasmid#232794PurposeCOMPASS Fragment 1 (Cps60, Cps50, Cps35, Cps25, Cps15) in PBIG1aDepositorInsertTagsNo tagsExpressionInsectPromoterpolyhedrin promoterAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoRHA
Plasmid#232994PurposeGalactose iduced expression of Gcn4 SATtoG KtoRHAin yeastDepositorInsertGcn4 SATtoG KtoR
Tags3xHA and TEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoR
Plasmid#232961PurposeGalactose iduced expression of Gcn4 ATtoG KtoR in yeastDepositorInsertGcn4 SATtoG KtoR
TagsTEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoRGFP
Plasmid#233006PurposeGalactose iduced expression of Gcn4 SATtoG KtoRGFP in yeastDepositorInsertGcn4 SATtoG KtoR
TagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 solvvol W
Plasmid#232977PurposeGalactose iduced expression of Gcn4 solvvol W+ in yeastDepositorInsertGcn4 solvvol W+
Tags3xHA and TEV cleavage siteExpressionYeastMutationS101T, D103E, S104T, D115E, N116Q, S117T, S122T, …PromoterGal1Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 SATtoG KtoR
Plasmid#231861PurposeBacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoRDepositorInsertGcn4 SATtoG KtoR
Tags6xHis, TEV cleavage siteExpressionBacterialMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterT7Available SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCY5h
Plasmid#160295PurposeYeast CRISPR plasmid targeting the hphMX cassetteDepositorInsertssgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9
UseCRISPRTags8xHis-tag and SV40 NLSAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCY5n
Plasmid#160296PurposeYeast CRISPR plasmid targeting the natMX cassetteDepositorInsertssgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9
UseCRISPRTags8xHis-tag and SV40 NLSAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-ANP1-FAPoptim
Plasmid#221120PurposeFAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)DepositorInsertANP1-FAPoptim
ExpressionYeastPromoterTEF1Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-ERG6-FAPoptim
Plasmid#221121PurposeFAP-tagged marker for lipid droplets (FAP optimized for yeast expression)DepositorInsertERG6-FAPoptim
ExpressionYeastPromoterTEF1Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-PMA1-FAPoptim
Plasmid#221122PurposeFAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)DepositorInsertPMA1-FAPoptim
ExpressionYeastPromoterTEF1Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only