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We narrowed to 61 results for: CCH

Showing: 1 - 20 of 61 results

  1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...created SRIRACCHA in 2017 to enrich and identify desired CRISPR mutants (Wen et al., 2018). SRIRACCHA uses...Madison lab also created an inducible SRIRACCHA system (iSRIRACCHA) where Cas9 expression is activated ...focus on two fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover single base-pair...editing CRISPR reporters in the near future.  SRIRACCHA (stable, but reversible, integrated reporter for...genome of mammalian cells.  Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017...compared to unsorted cells (Wen et al., 2018). Thus SRIRACCHA enables scientists to both quantify Cas9 activity...you with just your desired genomic edit.  Find SRIRACCHA plasmids at Addgene GEMCherry2, an improved monomeric...

  2. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org...antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact, 12 :96. https:...

  3. Microbiology Resources

    Type
    Collection
    ...application and by color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (...Reagent Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window...

  4. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...methionine synthesis (5). The budding yeast, Saccharomyces. cerevisiae, makes a great reference organism... or not proteins from the fission yeast, Schizosaccharomyces pombe, have the same function as their counterparts...yeast for budding geneticists: A primer on the Saccharomyces cerevisiae model system. Genetics 197, 33-48...yeast for young geneticists: A primer on the Schizosaccharomyces pombe model system. Genetics 201, 402-423...Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 61, 503-532.... et al. Functional characterization of the Saccharomyces cerevisiae genome by gene deletion and parallel...

  5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M, Bacci ML, Polishchuk RS, Auricchio A (2015) Improved...genes into AAV First, some history. In 2008, the Auricchio lab decided to solve the issue of “tiny vector...Cutillo L, Sparrow JR, Williams DS, Bennett J, Auricchio A (2008) Serotype-dependent packaging of large..., Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective delivery of large genes to ...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    ...ultracentrifugation altogether. Chomczynski and Sacchi showed that RNA could be effectively separated ...method are still widely used today (Chomcynski and Sacchi, 2006).  View our protocol for RNA extraction! ...https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi N (1987) Single-Step Method of RNA Isolation by...doi.org/10.1016/0003-2697(87)90021-2 Chomczynski P, Sacchi N (2006) The single-step method of RNA isolation...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...PLoS One. 2014 Jun 5;9(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic...sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Biotechnol Biofuels...

  9. Kit Free RNA Extraction

    Type
    Protocol
    ... (Link opens in a new window) Chomczynski P and Sacchi N, 2006 and (Link opens in a new window) TRIzol...

  10. Five Popular Model Organisms

    Type
    Blog Post
    ...By Alyssa Cecchetelli and Lukas Morgan Model organisms are vital tools used by researchers around the... of the fly (Pfeiffer et al., 2010). Yeast (Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic....5 (2011): 190-195. Liu, Wei, et al. "From Saccharomyces cerevisiae to human: The important gene co‑expression...

  11. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    .... 5. Five Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms...Bacterial Conjugation, and Transfection by Alyssa Cecchetelli The introduction of nucleic acids into bacteria...

  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...primarily pertains to ORIs in budding yeast, Saccharomyces cerevisiae; however, we’ve also noted some features...required for the replication of fission yeast, Schizosaccharomyces pombe, vectors at the end.) In a previous...

  14. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ... degron (AID) system in C. elegans By Alyssa Cecchetelli The auxin inducible degron (AID) system has been...repression, and editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...very technically challenging to use. Alyssa D. Cecchetelli is a Scientist at Addgene. She received her PhD... Alyssa Cecchetelli...

  16. Plasmids for Stem Cell Research

    Type
    Collection
    ... Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged...

  17. Genetic Code Expansion

    Type
    Collection
    ...azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea Musacchio 127411 pEVOL-pylT-N346A/C348A PylRS M. mazei mono-substituted...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...pharmaceutical industry, depend on the yeast, Saccharomyces cerevisiae, as a key host for the production...designed to complement unused auxotrophies in Saccharomyces strains by compensating for histidine (HIS3)...

  20. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...tagged you it's because we love your Insta!! #LILCChristmas . . #lab #ladiesinlabcoats #Christmas #labchristmas...
Showing: 1 - 20 of 61 results