We narrowed to 6,969 results for: tet on
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Plasmid#205603PurposeTet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertionDepositorInsertγ-tubulinWT-RNAi-resistant-GFP
UseTagsGFPExpressionMammalianMutationG2228A, C2231T, C2237T, G2240APromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-TAB-mOvol1
Plasmid#120360PurposepiggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).DepositorInsertOvol1 (Ovol1 Mouse)
UsePiggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT047A
Plasmid#159408PurposeConstitutive LuxR expression, controlled under T7 bacteriophage promoter, and inducible PFO-sfGFP chimeric protein expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.T7-LuxR-pr.LuxR-sfGFP-PFO
UseSynthetic BiologyTagsFLAG/FLAGExpressionBacterialMutationPromotertet and pLux promoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_S176E
Plasmid#131203Purposemammalian expression, tet RDepositorInsertRIPK2 S176E (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationS176EPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_3K
Plasmid#131205Purposemammalian expression, tet RDepositorInsertRIPK2 K209/410/538R (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationK209/410/538RPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFP-RIP2_Y474F
Plasmid#131204Purposemammalian expression, tet RDepositorInsertRIPK2 Y474F (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationY474FPromoterpCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1 S245X
Plasmid#92372PurposeInducible expression of M1S245X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, S245XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRE/SP M1 N184X
Plasmid#92371PurposeInducible expression of M1 N184X spastin in mammalian cellsDepositorInsertspastin (SPAST Human)
UseTagsExpressionMammalianMutation1-194 bp of 5'UTR deleted, N184XPromoterTet-responsive P tightAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR
Plasmid#65091PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged sequence to render plasmid resistant to s…PromoterCMV, 2x TetAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS_KDR
Plasmid#65667PurposeMYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100E
Plasmid#65093PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 100 to glutamic acid (KDR backgrou…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_F65A
Plasmid#65092PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchange phenylalanine 65 to alanine (KDR backbone)PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_K36E_R38E
Plasmid#65095PurposeDestination vector with RBPMS with K36E/R38E mutation for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchange lysine 36 to glutamic acid and arginine 38…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_K100A
Plasmid#65663PurposeFLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 100 to alanine (KDR background)PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_K36E_R38A_E39A
Plasmid#65658PurposeFLAG-HA expression vector with RBPMS carrying mutation K36E, R38A, E39ADepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 36 to glutamic acid, arginine 38 t…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_K36A_R38A_E39A
Plasmid#65657PurposeFLAG-HA expression vector with RBPMS carrying mutation K36A, R38A, E39ADepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged lysine 36 to alanine, arguing 38 to alani…PromoterCMV, 2x TetAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_F27A
Plasmid#65661PurposeFLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationchanged phenylalanine 27 to alanine (KDR backgrou…PromoterCMV, 2x TetAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRT006.3 L1 dual-luciferase AI retrotransposition reporter
Plasmid#213031PurposeBi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integrationDepositorInsertTet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
UseLuciferaseTagsFirefly luciferase antisense intron (3' UTR)ExpressionMammalianMutationPromoterpTRE bidirectional tet-onAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR
Plasmid#111177PurposeTet-ON miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG
Plasmid#70183PurposeInducible expression of guide RNA with fluorescent GFP reporterDepositorInsertH1-Tet-sgrna cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only