We narrowed to 1,459 results for: Mbp

1. pET-FLAG-eSpCas9-plus

   Plasmid

   #126774

   Purpose

   Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMTTH+mEGFP-LANA-H1.4CTD

   Plasmid

   #157802

   Purpose

   Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   mEGFP-LANA-H1.4CTD (H1-4 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

   Plasmid

   #101208

   Purpose

   Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, N497A, R661A and Q695A

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Kv7.4 AB (325-557, Δ 368-523)/pET28HMT

   Plasmid

   #111052

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AB (325-557, Δ 368-523)

   Promoter

   T7

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHTSHP-Smt-IIShhN

   Plasmid

   #121135

   Purpose

   Expresses N-terminal SUMO tagged II-ShhN in bacteria

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Shh (Shh Mouse)
   

   Use

   Tags

   HIS, HRV3C site, MBP, and Smt tag

   Expression

   Bacterial

   Mutation

   Amino acid sequence from 26-195, with two extra i…

   Promoter

   tac

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

   Plasmid

   #101232

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   G528A, V583A, E584A, D585A, N588A and Q926A

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

   Plasmid

   #101229

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/S355C/S867C/N692A/M694A/Q695A/H698A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, S355C, S867C, N692A, M694A, Q695A an…

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. hzHDAC10

   Plasmid

   #225542

   Purpose

   Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.

   Depositor

   David Christianson

   Article

   Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21

   Insert

   Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
   

   Use

   Tags

   His6-MBP tag with cleavable TEV site

   Expression

   Bacterial

   Mutation

   Mutated A24E and D94A

   Promoter

   T7

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-FLAG-B-eSpCas9

   Plasmid

   #126772

   Purpose

   Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A; K1003A; R1060A; amino acids 1005-1013 repl…

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMAL c2x Tral FL-15A

    Plasmid

    #113011

    Purpose

    For bacterial expression of MBP fusion of full-length Drosophila Tral with phosphomutations (15A)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length tral (tral Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    15 PNG phosphorylation sites mutated to A

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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