3,636 results

1. pU6-chiRNA_dCrkgRNA2

   Plasmid

   #131140

   Purpose

   Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   dCrk gRNA1 (Crk Fly)
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUAS attB V5 dDispatched WT HA

   Plasmid

   #126411

   Purpose

   Expression of wild type dDispatched HA in Drosophila.

   Depositor

   Stacey Ogden

   Article

   Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

   Insert

   d-Dispatched1 (disp Fly)
   

   Tags

   HA

   Expression

   Insect

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUAS-attB V5 dDispatched delta CS HA

   Plasmid

   #126412

   Purpose

   Expression of V5 dDispatched Delta CS HA in Drosophila cells lines

   Depositor

   Stacey Ogden

   Article

   Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

   Insert

   d-Dispatched1 (disp Fly)
   

   Tags

   HA

   Expression

   Insect

   Mutation

   Disp1 Cleavage site

   Available Since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR_Trf2

   Plasmid

   #125159

   Purpose

   Gateway entry clone

   Depositor

   Julius Brennecke, Alexander Stark

   Article

   Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

   Insert

   Trf2 (Trf2 Fly)
   

   Use

   Gateway entry vector

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EBP50-pDONR221

   Plasmid

   #124243

   Purpose

   Gateway entry clone of Drosophila EBP50 cDNA

   Depositor

   Peter Vilmos

   Insert

   Drosophila EBP50 (CG10939 Fly)
   

   Expression

   Insect

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-Hemmar-DSCP-ftz-FLP(D)

   Plasmid

   #119894

   Purpose

   FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

   Depositor

   Rita Sousa-Nunes

   Article

   Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

   Inserts

   FLP(D)
   DSCP-ftz (ftz Fly)
   

   Use

   Drosophila expression

   Mutation

   a mutated form of FLP, which at position 5 contai…

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Fly 2.0 pIDTBlue

   Plasmid

   #117418

   Purpose

   Drosophila melanogaster gene cloned into pIDTBlue vector for internal extraction control

   Depositor

   Dirk Dittmer

   Insert

   FLY 2.0
   

   Tags

   NA

   Expression

   Bacterial

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dscam1_7.27.26 EC10-Fc-His

   Plasmid

   #72189

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.26
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Dscam1_7.27.23 EC10-Fc-His

   Plasmid

   #72187

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.23
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Dscam1_7.27.26 EC10-AP-His

    Plasmid

    #72063

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.26
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA[Tra].1026G

    Plasmid

    #112689

    Purpose

    express gRNA targeting Tra under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[Tra] (tra Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. gRNA[DsxF].1026A

    Plasmid

    #112690

    Purpose

    express gRNA targeting DsxF under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[DsxF] (dsx Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNAs[bTub+Tra].1046D

    Plasmid

    #112693

    Purpose

    express two gRNA targeting bTub & Tra under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. gRNA[bTub+DsxF].1046A

    Plasmid

    #112694

    Purpose

    express two gRNA targeting bTub & DsxF under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTGC-U6.3

    Plasmid

    #112810

    Purpose

    PCR template vector for amplifying gRNAcore-pU6.2 promoter fragment

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Insert

    U6:3-gRNAcore
    

    Expression

    Bacterial

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs_FLAG-Sfxn2 (CG6812)

    Plasmid

    #110643

    Purpose

    Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    CG6812 (CG6812 Fly)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs_FLAG-Sfxn1-3 (CG11739)

    Plasmid

    #110642

    Purpose

    Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    CG11739 (CG11739 Fly)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. GST-GNU-N

    Plasmid

    #112972

    Purpose

    Expresses fusion of GST and N terminal amino acids of GNU

    Depositor

    Terry Orr-Weaver

    Article

    Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

    Insert

    gnu (gnu Fly)
    

    Expression

    Bacterial

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. mei-S332 5.6Kb genomic DNA in Casper4

    Plasmid

    #112984

    Purpose

    P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

    Insert

    mei-S332 (Sgo) (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2-amo

    Plasmid

    #113190

    Purpose

    Expresses amo in vertebrate cells

    Depositor

    Craig Montell

    Article

    Watnick et al Curr Biol. 2003 Dec 16;13(24):2179-84.

    Insert

    amo (Pkd2 Fly)
    

    Use

    In vitro transcription

    Expression

    Mammalian

    Mutation

    Y602H

    Promoter

    simian CMV IE94

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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