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pFast-dCry-V5His

Plasmid

#136240

Purpose

Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

DmCry

Tags

His and V5

Expression

Insect

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET28P_dCrk

Plasmid

#131145

Purpose

Expresses 6XHis::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHD-dsRed-attP_dCrk_flankingHRs

Plasmid

#131141

Purpose

Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Inserts

Crk Upstream Homology Region (Crk Fly)
Crk Downstream Homology Region (Crk Fly)

Use

CRISPR and Cre/Lox; Homology directed repair temp…

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA1

Plasmid

#131139

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-chiRNA_dCrkgRNA2

Plasmid

#131140

Purpose

Encodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

dCrk gRNA1 (Crk Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS attB V5 dDispatched WT HA

Plasmid

#126411

Purpose

Expression of wild type dDispatched HA in Drosophila.

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Tags

HA

Expression

Insect

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-attB V5 dDispatched delta CS HA

Plasmid

#126412

Purpose

Expression of V5 dDispatched Delta CS HA in Drosophila cells lines

Depositor

Stacey Ogden

Article

Stewart et al Elife. 2018 Jan 23;7. pii: 31678. doi: 1

Insert

d-Dispatched1 (disp Fly)

Tags

HA

Expression

Insect

Mutation

Disp1 Cleavage site

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_Trf2

Plasmid

#125159

Purpose

Gateway entry clone

Depositor

Julius Brennecke, Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

Trf2 (Trf2 Fly)

Use

Gateway entry vector

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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EBP50-pDONR221

Plasmid

#124243

Purpose

Gateway entry clone of Drosophila EBP50 cDNA

Depositor

Peter Vilmos

Insert

Drosophila EBP50 (CG10939 Fly)

Expression

Insect

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-Hemmar-DSCP-ftz-FLP(D)

Plasmid

#119894

Purpose

FLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intron

Depositor

Rita Sousa-Nunes

Article

Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

Inserts

FLP(D)
DSCP-ftz (ftz Fly)

Use

Drosophila expression

Mutation

a mutated form of FLP, which at position 5 contai…

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Fly 2.0 pIDTBlue

Plasmid

#117418

Purpose

Drosophila melanogaster gene cloned into pIDTBlue vector for internal extraction control

Depositor

Dirk Dittmer

Insert

FLY 2.0

Tags

NA

Expression

Bacterial

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.26 EC10-Fc-His

Plasmid

#72189

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.26

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.23 EC10-Fc-His

Plasmid

#72187

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.23

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.26 EC10-AP-His

Plasmid

#72063

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.26

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[Tra].1026G

Plasmid

#112689

Purpose

express gRNA targeting Tra under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[Tra] (tra Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[DsxF].1026A

Plasmid

#112690

Purpose

express gRNA targeting DsxF under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[DsxF] (dsx Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gRNAs[bTub+Tra].1046D

Plasmid

#112693

Purpose

express two gRNA targeting bTub & Tra under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] & U6.3-gRNA[Tra] (tra Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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gRNA[bTub+DsxF].1046A

Plasmid

#112694

Purpose

express two gRNA targeting bTub & DsxF under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub] & U6.3-gRNA[DsxF] (dsx Fly)

Use

CRISPR

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTGC-U6.3

Plasmid

#112810

Purpose

PCR template vector for amplifying gRNAcore-pU6.2 promoter fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

U6:3-gRNAcore

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-Sfxn2 (CG6812)

Plasmid

#110643

Purpose

Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG6812 (CG6812 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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