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pDONR223_DNASE2_p.Y182H

Plasmid

#81374

Purpose

Gateway Donor vector containing DNASE2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DNASE2 (DNASE2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Y182H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXO15_p.E410K

Plasmid

#81405

Purpose

Gateway Donor vector containing FBXO15 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXO15 (FBXO15 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E410K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.G12A

Plasmid

#81277

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway entry vector

Tags

Expression

Mutation

G12A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTCF_p.N568K

Plasmid

#81612

Purpose

Gateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTCF (CTCF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

N568K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARAF_p.S214F

Plasmid

#82901

Purpose

Gateway Donor vector containing ARAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ARAF (ARAF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

S214F

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARAF_WT

Plasmid

#82096

Purpose

Gateway Donor vector containing ARAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARAF (ARAF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_A4GALT_WT

Plasmid

#81835

Purpose

Gateway Donor vector containing A4GALT , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

A4GALT (A4GALT Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ING1_WT

Plasmid

#82213

Purpose

Gateway Donor vector containing ING1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ING1 (ING1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ASCL4_WT

Plasmid

#81909

Purpose

Gateway Donor vector containing ASCL4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ASCL4 (ASCL4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDKN1B_p.C29F

Plasmid

#81307

Purpose

Gateway Donor vector containing CDKN1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDKN1B (CDKN1B Human)

Use

Gateway entry vector

Tags

Expression

Mutation

C29F, V109G

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.P367R

Plasmid

#82851

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

P367R

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAX_p.R60del

Plasmid

#82740

Purpose

Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R60del

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAX_p.R35L

Plasmid

#82732

Purpose

Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R35L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.W450L

Plasmid

#82830

Purpose

Gateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

W450L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag PAPSS1

Plasmid

#20557

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PAPSS1 (PAPSS1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
MKC106_HBB(HBA1reg-HBA1-IRES-tEPOR)

Plasmid

#232411

Purpose

AAV production plasmid for HBA+tEPOR vector from Figs. 4-6 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA+tEPOR cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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MKC62_HBB(HBA1reg-HBA1-2A-YFP)

Plasmid

#232408

Purpose

AAV production plasmid for HBA1-2A-YFP vector from Fig. 2 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank full HBA1-2A-YFP cassette.

Depositor

Kyle Cromer

Article

Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

Insert

Hemoglobin Subunit Alpha 1 (HBA1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk1_#1-puro

Plasmid

#231984

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk1_#2-puro

Plasmid

#231983

Purpose

Knockout mouse Ripk1

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-4gRNA-GBX2-RFP

Plasmid

#192288

Purpose

Expresses RFP and four sgRNAs against GBX2

Depositor

Mark Denham

Article

Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

Insert

RFP and four sgRNA GBX2 (GBX2 Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-RPS14(WT)-Myc

Plasmid

#122236

Purpose

expresses RPS14 protein with a Myc tag in mammalian cells (hygro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARSI_WT

Plasmid

#81898

Purpose

Gateway Donor vector containing ARSI , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARSI (ARSI Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GPR141_WT

Plasmid

#81876

Purpose

Gateway Donor vector containing GPR141 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GPR141 (GPR141 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CNBD1_WT

Plasmid

#81877

Purpose

Gateway Donor vector containing CNBD1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CNBD1 (CNBD1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_HERPUD2_WT

Plasmid

#81880

Purpose

Gateway Donor vector containing HERPUD2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HERPUD2 (HERPUD2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

A108T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_IKZF5_WT

Plasmid

#81855

Purpose

Gateway Donor vector containing IKZF5 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKZF5 (IKZF5 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

M204V; p.415_418delQHNQ; p.419_420-insITNID

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_C20orf72_WT

Plasmid

#81806

Purpose

Gateway Donor vector containing C20ORF72 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

C20orf72 (MGME1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_GZMA_WT

Plasmid

#81792

Purpose

Gateway Donor vector containing GZMA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GZMA (GZMA Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Y5N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_HRH4_WT

Plasmid

#81786

Purpose

Gateway Donor vector containing HRH4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRH4 (HRH4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_KRT26_WT

Plasmid

#81763

Purpose

Gateway Donor vector containing KRT26 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRT26 (KRT26 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge