We narrowed to 2,753 results for: PGK
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Plasmid#227753PurposeExpresses a version of light-inducible transcription factor EL222 (Gly94Cys) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationGly94Cys mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG120
Plasmid#227752PurposeExpresses a version of light-inducible transcription factor EL222 (Asp89Tyr) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationAsp89Tyr mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG137
Plasmid#227751PurposeExpresses a version of light-inducible transcription factor EL222 (Val201Leu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationVal201Leu mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG136
Plasmid#227750PurposeExpresses a version of light-inducible transcription factor EL222 (Pro165Ser) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationPro165Ser mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG135
Plasmid#227749PurposeExpresses a version of light-inducible transcription factor EL222 (Lys99Glu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationLys99Glu mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG119
Plasmid#227748PurposeExpresses a version of light-inducible transcription factor EL222 (Val139Leu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationVal139Leu mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG118
Plasmid#227747PurposeExpresses a version of light-inducible transcription factor EL222 (Asp31Tyr) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationAsp18Tyr mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIDAF
Plasmid#213708PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.DepositorInsertEGFP
TagsTwin-strep-tagExpressionMammalianPromoterhPGKAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-TST-EGFP-GPIBY55
Plasmid#213707PurposeFor lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.DepositorInsertEGFP
TagsTwin-strep-tagExpressionMammalianPromoterhPGKAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP8EFA
Plasmid#185854PurposeTesting a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
ExpressionYeastMutationDHFR P66LAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVG117
Plasmid#227746PurposeExpresses a version of light-inducible transcription factor EL222 (Asp31Tyr, Val139Leu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationAsp18Tyr, Val139Leu double mutantPromoterPGK1prAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-BTRC-MGMT_2
Plasmid#136416PurposeLentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(BTRC)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
wtUTR_FGFR1
Plasmid#252071PurposeFGFR1 CDS with FGFR1 5'UTRDepositorAvailable sinceSept. 21, 2026AvailabilityAcademic Institutions and Nonprofits only -
TEC_FGFR1
Plasmid#252072PurposeFGFR1 CDS with TEC 5'UTRDepositorAvailable sinceSept. 21, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEB2_15_Q-system
Plasmid#236221PurposeQ-system: Plasmid with QS under HHF2p, QF2 under PGK1p and mTurquoise2 under 5xQUAS_CYC1cp with homologous regions for genome integration into HO locus.DepositorInsertsQS, ENO2 terminator
QF2, ACT1 terminator
mTurquoise2, ADH1 terminator
UseSynthetic BiologyExpressionBacterialPromoter5xQUAS_CYC1cp, HHF2p, and PGK1pAvailable sinceMarch 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro
Plasmid#211821PurposePiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishmentDepositorInsertSpCas9-ABE8e, tracrRNA and Puromycin resistance gene
ExpressionMammalianPromoterchicken β-actin promoter, U6 promoter and hPGK pr…Available sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIALD2HMGr
Plasmid#185867PurposeKnocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiaeDepositorInsertALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
ExpressionYeastMutationNoneAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX305-N-dTAG-CARD8-ZUC
Plasmid#169990PurposeMammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1).DepositorAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dTAG NLRP1 P1214R
Plasmid#166826PurposedTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradationDepositorAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-Bcan-Ntrk1_4
Plasmid#136413PurposeLentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.DepositorInsertU6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only