We narrowed to 22,746 results for: Sele

1. pYPQ134-STU-As

   Plasmid

   #138103

   Purpose

   Golden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MRGPRX1-DuET

   Plasmid

   #213344

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MRGPRX1 (MRGPRX1 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUCmini-iCAP-PHP.B3

   Plasmid

   #103004

   Purpose

   non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B3 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCmini-iCAP-PHP.B2

   Plasmid

   #103003

   Purpose

   non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B2 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   Dec. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-GABARAPL1

   Plasmid

   #137759

   Purpose

   Expression of GABARAPL1

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   GABARAPL1 (GABARAPL1 Human)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIN-Ldha

   Plasmid

   #175939

   Purpose

   knockout mouse Ldha

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   Ldha gRNA (Ldha Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-myc-HLA-A24

   Plasmid

   #135507

   Purpose

   Mammalian expression of myc-tagged HLA-A*024:02

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*24:02 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKRG3-mU6-PUb-3xFLAG-hSpCas9

   Plasmid

   #162161

   Purpose

   Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

   Plasmid

   #211471

   Purpose

   lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   Cas9_T2A_mNeonGreen_P2A_BlasticidinR
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF-1a

   Available since

   March 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-FLuc

    Plasmid

    #83281

    Purpose

    recombinant AAV vector packaging single-stranded Firefly Luciferase under the CAG promoter (includes WPRE element)

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    Firefly Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGG-B1

    Plasmid

    #78504

    Purpose

    Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

    Depositor

    Miguel Sena-Esteves

    Article

    Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

    Inserts

    AAV2 Rep
    AAV-B1 capsid
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-MAP1LC3A

    Plasmid

    #137756

    Purpose

    Expression of LC3A

    Depositor

    Carol Mercer

    Article

    Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

    Insert

    MAP1LC3A (MAP1LC3A Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. aMHC-mCherry-Rex-Blasticidin

    Plasmid

    #21228

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-FLAG-APEX2-LGALS3

    Plasmid

    #175756

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-Mch-MAP1LC3B

    Plasmid

    #201924

    Purpose

    For lentiviral transduction of Mch-MAP1LC3B

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDB_053

    Plasmid

    #216083

    Purpose

    for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
    

    Use

    Destination

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-B-evoSpCas9-NLS-6xHis

    Plasmid

    #207390

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a Wb-iPGM

    Plasmid

    #180246

    Purpose

    Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    Wuchereria bancrofti iPGM-10His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. aMHC-eGFP-Rex-Neo

    Plasmid

    #21229

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    alpha myosin heavy chain promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQTEV-MAT2B

    Plasmid

    #34795

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    methionine adenosyltransferase II, beta (MAT2B Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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