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pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

Plasmid

#139788

Purpose

Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

Depositor

Arneet Saltzman

Publication

Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

Type

Empty backbone

Tags

6xHis (cleavable by TEV protease) and GST (cleava…

Expression

Bacterial

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5/FRT/TO C1-APEX2

Plasmid

#129641

Purpose

Express ER-localized C1-APEX2 fusion in mammalian cells

Depositor

Nicholas Ingolia

Publication

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

C1(1-29)-APEX2-GFP

Expression

Mammalian

Available since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-3xFLAG-MyD88-GyrB

Plasmid

#131350

Purpose

Expresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase B

Depositor

Jonathan Kagan

Publication

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

MyD88-GyrB (Myd88 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-TRAF6-targeting sgRNA 2

Plasmid

#131346

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Publication

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBJ1-rat Beta2m

Plasmid

#121670

Purpose

Expresses rat β-2-microglobulin in mammalian cells

Depositor

Pamela Bjorkman

Publication

Tesar et al Traffic. 2006 Sep;7(9):1127-42.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

rat beta-2- microglobulin (B2m Rat)

Expression

Mammalian

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Emerin

Plasmid

#120246

Purpose

Expresses V5-tagged Emerin in lentiviral vector

Depositor

Larry Gerace

Publication

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Emerin (Emd Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pmPB3-VSV

Plasmid

#68039

Purpose

Expresses mouse Plexin-B3 with N-terminal VSV tag in mammalian cells

Depositor

Roland Friedel

Publication

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Plexin-B3 (Plxnb3 Mouse)

Tags

VSV

Expression

Mammalian

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLJM1-PHD2

Plasmid

#223552

Purpose

Lentivirus transfer plasmid for expression of full length human PHD2

Depositor

Somshuvra Mukhopadhyay

Publication

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSR29

Plasmid

#69255

Purpose

pelt-2::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Publication

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0, tbb-2 UTR is in wrong o…

Promoter

elt-2

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pEG607

Plasmid

#40193

Depositor

Geraldine Seydoux

Publication

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (mex-5 Nematode)

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…

Expression

Worm

Promoter

4.4 kb mex-5 promoter fragment

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
PECAM1-mRuby3-T2A-secNluc

Plasmid

#235607

Purpose

Donor plasmid for knock-in of mRuby3-T2A-secNluc into the human PECAM1 locus

Depositor

Kai Wang

Publication

Zhao et al Angiogenesis. 2025 Jan 3;28(1):10. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PECAM1 homologous recombination arms with PuroR drug selection cassette

Use

CRISPR

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/GFPCD63

Plasmid

#231709

Purpose

Stably express CD63 fused with GFP in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Publication

Li et al Sci Rep. 2024 Nov 9;14(1):27432. doi: 10

Insert

CD63 (CD63 Human)

Tags

gfp

Expression

Mammalian

Promoter

CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-ngRNA+41_EF1a-puroR

Plasmid

#207358

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Publication

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 +41 ngRNA

Use

Lentiviral

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGMC00028 (aka pTS0057)

Plasmid

#200832

Purpose

AAVS1 guide RNA cloned into the lentiSAM puro backbone

Depositor

Raj Chari

Publication

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Insert

AAVS1 guide RNA

Use

Lentiviral

Expression

Mammalian

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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FAB(PX-LD)

Plasmid

#214426

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP-V5

Tags

CFAST-Halo and FKBP-V5-RspAN

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_Cre

Plasmid

#201198

Purpose

AAV vector for cre expression under EF1 promoter

Depositor

Tetsuo Yamamori

Publication

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Inserts

cre
EF1alpha promoter

Use

AAV

Expression

Bacterial and Mammalian

Mutation

nuclear targeting cre

Promoter

N/A and hEF1alpha promoter

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

Plasmid

#168173

Purpose

Expression of BeCyclOp in BWMs of C. elegans

Depositor

Alexander Gottschalk

Publication

Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

Insert

BeCyclOp (codon optimized for C. elegans)

Expression

Worm

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-TIS11B CPM2

Plasmid

#121163

Purpose

Mammalian expression of GFP tagged TIS11B CPM2 (charge pattern mutant 2)

Depositor

Christine Mayr

Publication

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ZFP36L1 (TIS11B) charge pattern mutant 2

Expression

Mammalian

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-DCX-2A-mCherry

Plasmid

#118290

Purpose

Expresses human DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

Depositor

Vance Lemmon

Publication

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

doublecortin (DCX Human)

Use

AAV

Tags

2A peptide and mCherry

Expression

Mammalian

Promoter

CMV with beta global intron

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-P2A-Puro

Plasmid

#110851

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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