We narrowed to 2,820 results for: pDONR221

Showing: 2701 - 2720 of 2820 results

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pME-EGFP-miR-MCS (JDW 1231)

Plasmid

#242562

Purpose

Gateway middle entry clone containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with artifial intron and MCS

Use

Gateway subcloning

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mRuby2-3xMyc (JDW 1246)

Plasmid

#242554

Purpose

Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mRuby2

Use

Gateway subcloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR221-ITGB1(no stop)

Plasmid

#215453

Purpose

Gateway entry vector with integrin beta1 wild-type (WT) without a stop codon

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

none

Available Since

Sept. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pME-zFUCCI (JDW 1464)

Plasmid

#242558

Purpose

Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mStayGold-WPRE (JDW 1384)

Plasmid

#242550

Purpose

Gateway middle entry clone containing H2B-mStayGold with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mStayGold (monomeric StayGold)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mBaoJin-WPRE (JDW 1383)

Plasmid

#242549

Purpose

Gateway middle entry clone containing H2B-mBaoJin with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mBaoJin

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mScarlet-3H (JDW 1513)

Plasmid

#242551

Purpose

Gateway middle entry clone containing H2B-mScarlet-3H; Nuclear red fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-H

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-mCerulean3-TUBB1A (JDW 1508)

Plasmid

#242555

Purpose

Gateway middle entry clone containing mCerulean3-TUBB1A; Cyan fluorescent reporter for visualizing tubulin in cells

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean3-TUBB1A

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-MCS (JDW 455)

Plasmid

#229832

Purpose

A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

attL1/L2 flanked MCS

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME-StayGoldc4-Giantin (JDW 1355)

Plasmid

#229844

Purpose

A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

n2StayGoldc4

Use

Gateway entry clone

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pME AmCyan (JDW 1151)

Plasmid

#224485

Purpose

Gateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

AmCyan

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mCherry-3xHA (JDW 1321)

Plasmid

#224493

Purpose

Gateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Lifeact-mCherry-3xHA

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-mCerulean3-TUBB1A (JDW 1367)

Plasmid

#224503

Purpose

Gateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cells

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean3-TUBB1A

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC (no LexOp) (JDW 937)

Plasmid

#224504

Purpose

Gateway compatible middle entry clone containing a destabilized LexA transactivator

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-myc-BirA (JDW 1210)

Plasmid

#224509

Purpose

Gateway compatible middle entry clone containing a Myc tagged BirA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BirA

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Myc-BioID2-MCS1 (JDW 1095)

Plasmid

#224510

Purpose

Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BioID2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Myc-BioID2-MCS2 (JDW 1209)

Plasmid

#224511

Purpose

A Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCS

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Myc-BioID2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME nls EGFP-Cre (JDW 1329)

Plasmid

#224515

Purpose

A Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusion

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nls-EGFP-Cre

Use

Cre/Lox

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pME H2B mCerulean (JDW 1150)

Plasmid

#224543

Purpose

A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mCerulean

Use

Gateway cloning

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pJW1827

Plasmid

#154323

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I

Use

CRISPR

Expression

Worm

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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