We narrowed to 10,133 results for: ada

1. pCIwt-PRM-Wasabi

   Plasmid

   #195553

   Purpose

   Always-on expression of mWasabi reporter with transcription activation by wildtype CI

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mWasabi
   CIwt
   

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCmini-NFKB1-PGK-EGFP

   Plasmid

   #204018

   Purpose

   Homologous donor template for NFKB1 knockout expressing EGFP

   Depositor

   Yohei Sato

   Article

   Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUCmini-NFKB1-PGK-tdTomato

   Plasmid

   #204019

   Purpose

   Homologous donor template for NFKB1 knockout expressing tdTomato

   Depositor

   Yohei Sato

   Article

   Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

   Insert

   tdTomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUCmini-NFKB2-PGK-EGFP

   Plasmid

   #204020

   Purpose

   Homologous donor template for NFKB2 knockout expressing EGFP

   Depositor

   Yohei Sato

   Article

   Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCmini-NFKB2-PGK-tdTomato

   Plasmid

   #204021

   Purpose

   Homologous donor template for NFKB2 knockout expressing tdTomato

   Depositor

   Yohei Sato

   Article

   Sato et al Heliyon. 2023 Nov 26;9(12):e22911. doi:

   Insert

   tdTomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUPD2-HR1bR181A R185A-GFP-L (B3-B4)

   Plasmid

   #211857

   Purpose

   Mutant E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Mutation

   R181A R185A

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)

   Plasmid

   #211863

   Purpose

   Nuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA

   Mutation

   C492A

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBbA5k-AcrAB-AsLOV2*(543)

   Plasmid

   #210863

   Purpose

   IPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.

   Depositor

   Mary Dunlop

   Article

   Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

   Inserts

   acrA
   acrB
   

   Use

   Synthetic Biology

   Tags

   AsLOV2*(543)

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR2_IHFdist_rm

   Plasmid

   #162318

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Expression

   Bacterial

   Mutation

   IHF distal site is removed

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-sTagRFP-Zeo

    Plasmid

    #207734

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. qTAG-N-Zeo-sTagRFP

    Plasmid

    #207701

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. qTAG-C-sTagRFP-Blast

    Plasmid

    #207712

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. qTAG-N-Puro-sTagRFP

    Plasmid

    #207690

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. qTAG-N-Blast-sTagRFP

    Plasmid

    #207679

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pCMV-PRLSS-yATOM_SH2-KDEL

    Plasmid

    #209714

    Purpose

    Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-yATOM_SH2-KDEL
    

    Tags

    PRLSS

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-MBP-yATOM_WDR5 (R80A)

    Plasmid

    #209713

    Purpose

    Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    MBP-yATOM_WDR5 (R80A)
    

    Tags

    MBP

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

    Plasmid

    #209718

    Purpose

    Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-NbGFP_miRFP670nano3-KDEL
    

    Tags

    PRLSS

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-PRLSS-Clover-KDEL

    Plasmid

    #209720

    Purpose

    Mammalian expression of clover localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-Clover-KDEL
    

    Tags

    PRLSS

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Cox4-yATOM_SH2

    Plasmid

    #209715

    Purpose

    Mammalian expression of y-ATOM biosensor for SH2 localized to mitochondria

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    Cox4-yATOM_SH2
    

    Tags

    Cos4

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-SNIFP

    Plasmid

    #197210

    Purpose

    Expresses the protein of SNIFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    SNIFP
    

    Use

    AAV

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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