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We narrowed to 14,168 results for: eng

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Showing: 2721 - 2740 of 14168 results

  3. bNS2B47NS3

    Plasmid
    #155320
    Purpose
    co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length
    Depositor
    Dahai Luo
    Publication
    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:
    Insert
    Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155320-plasmid-map-sequence-id-318477

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  5. pDIRECT_26H

    Plasmid
    #91150
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91150-plasmid-map-sequence-id-182284

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  7. pM-ABE

    Plasmid
    #196292
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding ABE in place of viral GP
    Depositor
    Zhenghe Li
    Publication
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding ABE in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196292-plasmid-map-sequence-id-390025

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  8. pGGG Wheat GUS marker

    Plasmid
    #165418
    Purpose
    Wheat transformation binary vector pGGG confers hygromycin resistance in wheat and contains the GUS (2x introns) reporter gene driven by OsUbi promoter
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Publication
    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.
    Insert
    Os Ubiquitin Promoter::Gus (2x introns)::NosT
    Use
    Wheat transformation binary vector
    Promoter
    OS UbiP::GUS (2xint)::NosT
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165418-plasmid-map-sequence-id-331269

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  16. pORTMAGE-1

    Plasmid
    #72680
    Purpose
    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.
    Depositor
    Csaba Pál
    Publication
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Tags
    none
    Expression
    Bacterial
    Mutation
    lower strength RBS before mutL E32K allele than i…
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  17. pSLS.492

    Plasmid
    #184957
    Purpose
    Test effect of a1/a2 length on editing rpoB using retron recombineering
    Depositor
    Seth Shipman
    Publication
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    Expression
    Bacterial
    Mutation
    rpoB donor T1534C, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184957-plasmid-map-sequence-id-376681

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  18. dCas12Max-CBE

    Plasmid
    #195337
    Purpose
    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Publication
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-CBE
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195337-plasmid-map-sequence-id-383131

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  20. pCgCas9_recT

    Plasmid
    #153337
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr
    Depositor
    Sheng Yang
    Publication
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153337-plasmid-map-sequence-id-305977

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Showing: 2721 - 2740 of 14168 results