We narrowed to 159,141 results for: addgene

1. pMSCVII

   Plasmid

   #162750

   Purpose

   MSCV backbone

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD379

   Plasmid

   #91834

   Purpose

   SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNM3725

   Plasmid

   #154113

   Purpose

   UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   UAS 11X::∆pes-10::GFP-C1::tbb-2 3'
   

   Use

   Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. 6CCR (GID4)

   Plasmid

   #119960

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   GID4 (C17orf39 Human)
   

   Use

   Tags

   6xHIS, thrombin,SUMO and TEV cleavage

   Expression

   Bacterial

   Mutation

   contains amino acids 116-300

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLenti SpBsmBI sgRNA Puro

   Plasmid

   #62207

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. 6BY9 (EHMT1)

   Plasmid

   #119956

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   EHMT1 (EHMT1 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. HO-pGAL-poly-KanMX4-HO

   Plasmid

   #51664

   Purpose

   yeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoter

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Yeast integration

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. ITCH

   Plasmid

   #36197

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ITCH (ITCH Human)
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains HECT domain

   Promoter

   Available since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pKL09-GCaMP6f-mScarlet bb118

   Plasmid

   #158979

   Purpose

   Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   GCaMP6f-mScarlet fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#100833

   Promoter

   Bba_J23118 (sigma 70)

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5KO9 (HMCES)

    Plasmid

    #101877

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HMCES (HMCES Human)
    

    Use

    Tags

    6x His (modified C-term tag is AENLYFQSHHHHHH)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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