We narrowed to 42,599 results for: cha;
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Plasmid#108691PurposeVTC4 sequence expressed in yeast plasmidDepositorInsertVacuolar Transporter Chaperone 4
UseTagsExpressionYeastMutationPromoterVTC4 (native promoter)Available sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKF-P14MMP2AG-T2APuroR-5h
Plasmid#128256PurposeMammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.DepositorInsertrtTA-Advanced::P2A::EGFP::T2A::PuroR
UseSynthetic Biology; Flp-in expression vectorTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mouse c-myc (exon I)
Plasmid#8446DepositorInsertMouse c-myc (exon I) (Myc Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(1234-1443)
Plasmid#35078DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionMutationContains aa 1234-1443PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
r-22. pT7-7-SNCA-9Asn
Plasmid#107297PurposePlasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 Asn and 13 GlyDepositorInsertSNCA-9Asn (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
t2. pT7-7-SNCA-5Lys
Plasmid#107294PurposePlasmid for the expression of the construct SNCA-5Lys. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Lys and 7 GlyDepositorInsertSNCA-5Lys (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
r-6. pT7-7-SNCA-2Asn
Plasmid#107296PurposePlasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 6 aa C-terminal moiety, which consists of 2 Asn and 4 GlyDepositorInsertSNCA-2Asn (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
r. pT7-7-SNCA-5Asn
Plasmid#107295PurposePlasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asn and 7 GlyDepositorInsertSNCA-5Asn (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
s2. pT7-7-SNCA-5Asp
Plasmid#107293PurposePlasmid for the expression of the construct SNCA-5Asp. Alpha-Synuclein with 12 aa C-terminal moiety, which consists of 5 Asp and 7 GlyDepositorInsertSNCA-5Asp (SNCA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3-2X
Plasmid#110835PurposeCMV expression vector for BE3-2X construct (not codon optimized)DepositorInsertBE3-2X
UseTagsExpressionMammalianMutation2 tandem NLS sequences in the XTEN linkerPromoterCMVAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWHI2-NAT
Plasmid#80588PurposeCEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoterDepositorInsertWHI2 (WHI2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterNative WHI2 promoterAvailable sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUDP010
Plasmid#101166PurposeE. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS1212
Plasmid#63785PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection markerDepositorInserteGFP-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseGFP-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-hRIP3-FL VV458/460PP
Plasmid#61368PurposeExpresses Human full length RIPK3 with A458P/V460P mutation in the mammalian cellsDepositorInsertFull length human RIP3 with VV458/460P mutation (RIPK3 Human)
UseTagsEYFPExpressionMammalianMutationchanged V458 to P and V460to PPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-miR-125b-1
Plasmid#58989PurposeMSCV based expression vector - co-expresses HcRed and human miR-125b-1DepositorInsertsUseRetroviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-hRIP1-FL I539P
Plasmid#61380PurposeExpresses Human full length RIPK1with I539P mutation in the mammalian cellsDepositorInsertFull length human RIP1with I539P mutation (RIPK1 Human)
UseTagsEGFP and HA tagExpressionMammalianMutationchanged I539 to PPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
BRCA1-(1057-1234)
Plasmid#35076DepositorInsertBRCA1 (BRCA1 Human)
UseTagsHisExpressionMutationContains aa 1057-1234PromoterT7Available sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe hygro human Frataxin
Plasmid#11504DepositorInsertFrataxin (FXN Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBabe neo human Frataxin
Plasmid#11503DepositorInsertFrataxin (FXN Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-cNM2A-Flag
Plasmid#236532PurposeFor expression of NM2A (dog) - with HA and FLAG tags in mammalian cellsDepositorInsertMYH9
UseTagsFLAG and HAExpressionMammalianMutationWTPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pRDA_052_HygroR
Plasmid#228937PurposePlasmid for cloning of custom gRNA using EnAsCas12aDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Hyg-ADE2
Plasmid#232104PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Kan-ADE2
Plasmid#232105PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgMTHFD1
Plasmid#217436PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human MTHFD1DepositorInsertsgRNA targeting MTHFD1 (MTHFD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLJC2-TK2-3xFLAG
Plasmid#217427PurposeLentiviral overexpression of human TK2DepositorInsertTK2 (TK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationInsert sequence is a codon-optimized gBLOCK (IDT)PromoterCMVAvailable sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgTYMS
Plasmid#217430PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human TYMSDepositorInsertsgRNA targeting TYMS (TYMS Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgTK2_4
Plasmid#217431PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human TK2DepositorInsertsgRNA 4 targeting TK2 (TK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgTK2_5
Plasmid#217432PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human TK2DepositorInsertsgRNA 5 targeting TK2 (TK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgSHMT2
Plasmid#217435PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human SHMT2DepositorInsertsgRNA targeting SHMT2 (SHMT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB222
Plasmid#221772PurposeBacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPasesDepositorInsertArfGEF1 (ARFGEF1 Human)
UseTags10xHis-SUMOExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB8589
Plasmid#175221PurposeXenopus oocyte expression vector containing YMR253C. Contains T7 RNA polymerase binding site, and adds polyA tail to YMR253C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYMR253C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8707
Plasmid#175222PurposeXenopus oocyte expression vector containing YNL095C. Contains T7 RNA polymerase binding site, and adds polyA tail to YNL095C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYNL095C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8728
Plasmid#175223PurposeXenopus oocyte expression vector containing YOL060C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL060C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOL060C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8711
Plasmid#175225PurposeXenopus oocyte expression vector containing YOR092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR092W
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8713
Plasmid#175226PurposeXenopus oocyte expression vector containing YOR271C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR271C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR271C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8590
Plasmid#175227PurposeXenopus oocyte expression vector containing YOR307C. Contains T7 RNA polymerase binding site, and adds polyA tail to YOR307C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYOR307C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8738
Plasmid#175228PurposeXenopus oocyte expression vector containing YPL189W. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL189W. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYPL189W
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8591
Plasmid#175229PurposeXenopus oocyte expression vector containing YPL244C. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL244C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYPL244C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8592
Plasmid#175230PurposeXenopus oocyte expression vector containing YPL264C. Contains T7 RNA polymerase binding site, and adds polyA tail to YPL264C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYPL264C
UseMrna expression, injection into xenopus oocytesTagsPolyAExpressionMutationPromoterT7Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only