MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals

Lentiviral vector for Gal4 inducible leucinezipper Pembrolizumab and constitutive mCherry expression

Lentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expression

Ready-to-use AAV PHP.eB particles produced from pAAV_BiCHATe27_dTomato_nlsdTomato (#213829). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the cholinergic neuron-targeting enhancer E27. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

Ready-to-use AAV PHP.eB particles produced from pAAV_BiCHATe27_ChR2_mCherry (#213830). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the cholinergic neuron-targeting enhancer E27. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

mini CRISPRcharm expression in mammalian cells

CRISPRcharm expression in mammalian cells

AAV construct expressing dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_dTomato_nlsdTomato

CRISPRcharm Kv1 expression in mammalian cells

A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.

A plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

A plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.

CRISPRcharm Kv2 expression in mammalian cells

AAV construct expressing HA-hM3Dq-dTomato driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_Gq_dTomato

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_ChR2_mCherry

AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

AAV construct expressing GCaMP6f driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_GCaMP6f

AAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_C1V1_eYFP