We narrowed to 68,460 results for: nin

1. pSPIH6

   Plasmid

   #190676

   Purpose

   E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)

   Depositor

   John Vederas

   Article

   Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi

   Inserts

   Small ubiquitin-like modifier
   Mxe GyrA intein with C-terminal chitin binding domain and His tag
   

   Tags

   Chitin binding domain, His tag, and N-terminal Hi…

   Expression

   Bacterial

   Mutation

   Added C-terminal his tag and None

   Promoter

   T7

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AXBEv2

   Plasmid

   #204606

   Purpose

   Mammalian expression of evolved adenine transversion base editor AXBEv2

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1a-GalT-IRES-Puromycin

   Plasmid

   #134862

   Purpose

   Expresses EGFP-tagged Golgi marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   beta 1,4-galactosyltransferase (B4GALT1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 83-398

   Promoter

   EF1a

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-mScarlet-I-P2A-iCre (JDW 917)

   Plasmid

   #224516

   Purpose

   A Gateway-compatible middle entry clone containing mScarlet-I P2A-iCre (codon optimized)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet-I-P2A-iCre
   

   Use

   Cre/Lox

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Neo-Bam APC 1-1941

   Plasmid

   #16510

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Expression

   Mammalian

   Mutation

   Codon 1941 truncation

   Available Since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-Neo-Bam APC 1-2644

   Plasmid

   #16511

   Depositor

   Bert Vogelstein

   Article

   Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

   Insert

   APC (APC Human)
   

   Expression

   Mammalian

   Mutation

   Codon 2644 truncation

   Available Since

   April 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH-ABE8e

   Plasmid

   #200774

   Purpose

   For plant adenine base editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

   Insert

   nCas9(D10A)-TadA8e
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…

   Promoter

   maize Ubiquitin-1, OsU3

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ACBE

   Plasmid

   #204607

   Purpose

   Mammalian expression of adenine-to-cytosine base editor ACBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-mTurquoise-P2A-iCre (JDW 920)

   Plasmid

   #224518

   Purpose

   A Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mTurquoise-P2A-iCre
   

   Use

   Cre/Lox

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-Neo-Bam APC 1-331

    Plasmid

    #16508

    Depositor

    Bert Vogelstein

    Article

    Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

    Insert

    APC (APC Human)
    

    Expression

    Mammalian

    Mutation

    Codon 331 truncation

    Available Since

    April 30, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pSIL-eGFP-shACTN1

    Plasmid

    #52676

    Purpose

    shRNA against α-actinin-1 with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Insert

    ACTN1 (Actn1 Rat)
    

    Use

    RNAi

    Tags

    CMV-EGFP

    Promoter

    U6

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. AMA1-bio

    Plasmid

    #47741

    Purpose

    Expresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised AMA1
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

    Plasmid

    #201683

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-sgRNA-lib 2.0

    Plasmid

    #89638

    Purpose

    sgRNA expression in mammalian cells after Gateway cloning

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. HeLa full-length MLCK-GFP

    Plasmid

    #46316

    Depositor

    Anne Bresnick

    Article

    Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

    Insert

    HeLa long myosin light chain kinase (MYLK Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA6-N-3XFLAG-Vcp

    Plasmid

    #123600

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    valosin containing protein (Vcp Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. MRGPRX1-DuET

    Plasmid

    #213344

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRX1 (MRGPRX1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

    Plasmid

    #224490

    Purpose

    Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Golgi-mTagBFP-P2A-H2A-iRFP
    

    Use

    Gateway cloning

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCD5-PR8-TEV-sfGFP-twinStrep

    Plasmid

    #157933

    Purpose

    Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.

    Insert

    A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
    

    Tags

    sfGFP & TwinStrep

    Expression

    Mammalian

    Mutation

    E190D

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHD-SPARC2-S-LexA::p65

    Plasmid

    #133564

    Purpose

    Swap out effector and terminator to generate SPARC2-S CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    LexA::p65
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    20X UAS

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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