We narrowed to 36,658 results for: ELL

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CRISPR-StAR2 (Retro)

Plasmid

#222688

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR-StAR3 (Retro)

Plasmid

#222689

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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p931-SWITCH-OVER insert: EF1a-Blasti

Plasmid

#217890

Purpose

insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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p213-SWITCH-OFF

Plasmid

#217888

Purpose

Retroviral Switch-OFF vector for sgRNA expression; U6-BbsIx2-SWITCH-OFF-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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p204-U6-Switch-ON

Plasmid

#217883

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-ABCF2_STOP

Plasmid

#221425

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

Insert

ABCF2 (ABCF2 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRetro-UMI

Plasmid

#222686

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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p223-Pulse-Switch

Plasmid

#217889

Purpose

Lentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pNTK Gatad2a flox

Plasmid

#110812

Purpose

Donor targeting vector for generating mouse Gatad2a conditional knockout allele

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Use

Unspecified

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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px330 Gatad2a sgRNA (for deletion)

Plasmid

#110814

Purpose

Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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px330 Gatad2a sgRNA (for flox targeting)

Plasmid

#110815

Purpose

Used with pNTK Gatad2a flox allele targeting construct in mouse cells

Depositor

Jacob Hanna

Article

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Insert

mGataD2a (Gatad2a Mouse)

Expression

Mammalian

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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CD40L-SrtA

Plasmid

#121167

Purpose

pMP71 vector expressing Tomato - P2A - CD40L - SrtA - flag

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

CD40L-SrtA

Use

Retroviral

Tags

Tomato and flag

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 ΔEMV-6MT

Plasmid

#41737

Depositor

Raphael Kopan, Jeffrey Nye

Article

Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

Insert

Notch-1 Extracellular-domain-deleted construct (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 protein fragment lacking …

Available Since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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Flag‐hTim1

Plasmid

#49207

Purpose

expresses Flag-tagged human Tim1

Depositor

Lawrence Kane

Article

de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

Insert

Tim1 (HAVCR1 Human)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

Tim1 lacks its own signal sequence

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only
pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-EGFP-NLS-3xFlag

Plasmid

#132397

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1058A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-eGFP-W

Plasmid

#126686

Purpose

Constitutive, ubiquitous GFP expression.

Depositor

Darrell Kotton

Article

Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

Insert

eGFP

Use

Lentiviral

Promoter

EF1aL

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSIN-TRE-H2BeGFP-rtTA2

Plasmid

#165494

Purpose

Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

Depositor

Hector G. Palmer

Article

Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

Insert

Human histone H2B (H2BC21 Human)

Use

Lentiviral

Tags

EGFP

Mutation

-

Promoter

TetO-CMV

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 ICv-6MT

Plasmid

#41730

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Intracellular Domain (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 protein beginning at Vali…

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)

Plasmid

#163055

Purpose

Grx1-roGFP2 glutathione redox sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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