We narrowed to 19,374 results for: REN

1. lenti-EF1a-dCas9-KRAB-Puro

   Plasmid

   #99372

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-KRAB-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiGuide-Hygro-eGFP

   Plasmid

   #99375

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiGuide-Hygro-mTagBFP2

   Plasmid

   #99374

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mClY-N1

   Plasmid

   #90457

   Purpose

   A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

   Depositor

   Joseph Santos-Sacchi

   Article

   Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

   Insert

   mClY-N1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

   Promoter

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-Hygro-dTomato

   Plasmid

   #99376

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A dTomato from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-dTomato
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AbVec2.0-mIgkc

   Plasmid

   #127157

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Igkc (Igkc Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-EF1a-dCas9-VP64-Puro

   Plasmid

   #99371

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-VP64-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

   Plasmid

   #90332

   Purpose

   CRISPR interference

   Depositor

   Dirk Heckl

   Article

   Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

   Inserts

   dCas9
   na
   SFFV
   P2A-BSD
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AbVec2.1-mIglc2

   Plasmid

   #127156

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant region

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Iglc2 (Iglc2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AbVec2.0-mIghg2b

    Plasmid

    #127155

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

    Depositor

    Hedda Wardemann

    Article

    Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

    Insert

    Ighg2b (Ighg2b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pExodus CMV.Artemis

    Plasmid

    #40211

    Purpose

    Mammalian expression of codon-optimized Artemis.

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

    Insert

    Artemis (DCLRE1C Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM1 TMEM192-Flag-FKBP

    Plasmid

    #112743

    Purpose

    express TMEM192-Flag-FKBP

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    TMEM192-Flag-FKBP (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    Flag, FKBP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2-elavl3-H2B-GCaMP6s

    Plasmid

    #59530

    Purpose

    Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    H2B
    GCaMP6s
    

    Use

    Transposable element

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLDLR-Luc mutSRE

    Plasmid

    #14945

    Depositor

    Axel Nohturfft

    Article

    Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    LDLR promoter mut SRE (LDLR Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Point mutation in the SRE-1 sequence of the LDL r…

    Promoter

    Available since

    May 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-FLAG-SOX4

    Plasmid

    #110360

    Purpose

    Mammalian expression of FLAG-tagged SOX4

    Depositor

    Carlos Moreno

    Article

    Liu et al Cancer Res. 2006 Apr 15;66(8):4011-9. do

    Insert

    SOX4 (SOX4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    lacking 5' UTR

    Promoter

    Available since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiGuide-Hygro-iRFP670

    Plasmid

    #99377

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Inserts

    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a and hU6

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Epac-S-H74

    Plasmid

    #170338

    Purpose

    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2011 Apr 29;6(4):e19170. doi:

    Insert

    mTDel_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-UbC

    Plasmid

    #62806

    Purpose

    To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

    Depositor

    Pantelis Tsoulfas, Jae Lee

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Type

    Empty backbone

    Use

    AAV; Eucaryotic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1 FLCN (WT) FLAG GFP

    Plasmid

    #164978

    Purpose

    Lentiviral vector for FLCN expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FLCN (FLCN Human)
    

    Use

    Lentiviral

    Tags

    1xFLAG-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-Zdk1-EB1C

    Plasmid

    #107696

    Purpose

    C-terminal half of π-EB1, EGFP-tagged, dissociates from microtubule ends in blue light

    Depositor

    Torsten Wittmann

    Article

    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP and Zdk1

    Expression

    Mammalian

    Mutation

    EB1 aa 186-268

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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