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Plasmid#175088PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationS644APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2602
Plasmid#175086PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationT424A, T438APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2603
Plasmid#175087PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationT504A, T508APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-DM-HA-GFP11-KDEL
Plasmid#177723PurposeDM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFlucDM
UseTagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP- Ura
Plasmid#177732PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA markerDepositorInsertFlucDM
UseTagsHA, HDEL, KAR2 Signal Sequence, and eGFPExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0233M
Plasmid#178415PurposeReverse compl seq of pOME0134 flanked by Kpn and EcoRI for insertion into pcDNA3.1-DepositorInsertReverse complement of pOME0134 upstream MIR-SYN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0232M
Plasmid#178414PurposeREVERSE complement seqeunce KSHV Downstream Mir-SYN RNA IN PCDNA3.1-DepositorInsertKSHV Reverse complement of downstream MIR-SYN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His-GBAi W211A
Plasmid#171756PurposeFor the expression of His-GBAi W211A in bacteriaDepositorInsertGBAi
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010A
Plasmid#176078PurposepBac-10xUAS-L596_g9608-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertL596_g9608.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010B
Plasmid#176079PurposepBac-10xUAS-L596_g25050-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertL596_g25050.t1
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010C
Plasmid#176080PurposepBac-10xUAS-Ce FAR-8-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertCe FAR-8
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1010D
Plasmid#176081PurposepBac-10xUAS-Anccey_04371-GGS4-HAx3-p10-UTR-Opie2-dsRedDepositorInsertCe FAR-8
UseTags3xHAExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGlomyc-Ovol1
Plasmid#172871PurposeMammalian expression vector with myc-tagged mouse Ovol1 coding sequenceDepositorInsertOvol1 (Ovol1 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-SPN_ARR N52R (Shank3)
Plasmid#175255PurposeSPN-ARR fragment with structure opening N52R mutation in EGFP backbone for mammalian expressionDepositorInsertSPN-ARR fragment with N52R mutation from SHANK3
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_L139*
Plasmid#174400PurposeS. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130DepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationL139* (Truncation after 139th amino acid in sarA)PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_S130A
Plasmid#174399PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its GSK3-binding SxxxSP motif mutated to SxxxAPDepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationS130APromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Flag-stm2585_Y167F
Plasmid#174398PurposeNative S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags and its STAT3-binding YxxQ motif mutated to FxxQDepositorInsertsarA
UseTagsEGFP and FlagExpressionMammalianMutationY167FPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585-gp130
Plasmid#174393PurposeChimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.DepositorInsertsarA-IL6ST chimera
UseTagsFlagExpressionMammalianMutation140-177 of sarA replaced with homologous IL6ST se…PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585_Y167F
Plasmid#174392PurposeS. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQDepositorInsertstm2585
UseTagsFlagExpressionMammalianMutationTyr 167 changed to PhePromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV BARK R106A p2A mCherry
Plasmid#117688PurposeCMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencingDepositorInsertBARKrgs R106A peptide
UseTagsHA / FLAGExpressionMammalianMutationR106APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(2)-CRY2-mCherry
Plasmid#160441PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(1)-CRY2-mCherry
Plasmid#160440PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-attB2-SA(0)-CRY2-mCherry
Plasmid#160439PurposeTo create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.DepositorInsertCryptochrome 2 (CRY2 Mustard Weed)
UseTagsmCherryExpressionInsectMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Cre sgUroD v4
Plasmid#158036Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroDDepositorInsertUroD sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO-Cre sgItgb5 v3
Plasmid#158038Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5DepositorInsertItgb5 sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO-Cre sgRipk4 v3
Plasmid#158035Purposelenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4DepositorInsertRipk4 sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
HBT-CPK32ac-Flag
Plasmid#107431PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorInsertCPK32 (CPK32 Mustard Weed)
UseTagsFlagExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
PTPRA_C433S-SHC
Plasmid#139612PurposeStrepIII-HA-tagged gene expressionDepositorInsertPTPRA_C433S (PTPRA Human)
UseTagsStrepIII-HAExpressionMammalianMutationC433SPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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