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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 39,902 results for: KAN;

Showing: 6951 - 7000 of 39902 results

  23. t206-405

    Plasmid
    #166132
    Purpose
    For expression of human talin-1 head fragment (residues 206-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-F2F3(206-405) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 206-405 only
    Available Since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    166132-plasmid-map-sequence-id-330268

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  24. t1-405(del37/GAG)

    Plasmid
    #166129
    Purpose
    Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(del37/GAG) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    aa1-405, with 37 aa deletion in the F1-loop which…
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166129-plasmid-map-sequence-id-330258

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  44. ABL1-N-HiBiT kinase domain_v1

    Plasmid
    #207121
    Purpose
    Bacterially expressed HiBiT-ABL1 kinase domain (D233-S500) for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human Abelson Kinase Domain N-terminal HiBiT, N-terminal 10X His-tag (ABL1 Human)
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207121-plasmid-map-sequence-id-422565

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  46. t1-405(del30)

    Plasmid
    #166128
    Purpose
    Expression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(del30) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405, with 30 amino acid deletion in th…
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166128-plasmid-map-sequence-id-330260

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  47. t1-405(T144E,T150E)

    Plasmid
    #166130
    Purpose
    For expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    HuT1head1-405(T144E,T150E) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405, mutations T144E and T150E
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166130-plasmid-map-sequence-id-330250

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  48. Human/mouse ELL2

    Plasmid
    #127254
    Purpose
    Expresses a human/mouse chimera that increases ELL2 expression in mammalian cells
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    N-terminal human/C-terminal mouse ELL2 (ELL2, Ell2 Human, Mouse)
    Use
    Other
    Tags
    His6X
    Expression
    Mammalian
    Mutation
    N terminal amino acids (1-287) are human, C termi…
    Promoter
    pEF1a
    Available Since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    127254-plasmid-map-sequence-id-250057

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Showing: 6951 - 7000 of 39902 results