We narrowed to 7,283 results for: Tet-on

1. T7 promoter + filler DNA reporter plasmid

   Plasmid

   #156454

   Purpose

   A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

   Inserts

   NeoR/KanR
   TcR
   

   Expression

   Bacterial

   Mutation

   ATG start codon mutated to ACG

   Promoter

   Em7 and tet

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRelA'

   Plasmid

   #175595

   Purpose

   Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Catalytic domain of E.coli relA gene.
   

   Expression

   Bacterial

   Mutation

   Only the catalytic domain of the enzyme is used (…

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO myc hSeparase

   Plasmid

   #59820

   Purpose

   Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Dox-Akt

   Plasmid

   #207328

   Purpose

   PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)

   Depositor

   Jared Toettcher

   Article

   Underhill et al Development. 2023 Aug 15;150(16):dev2016

   Insert

   rac protein kinase-alpha (AKT1 Human)
   

   Tags

   myristoylation tag - BFP

   Expression

   Bacterial

   Mutation

   range: 129 - 480

   Promoter

   Tet Response Element

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICE-EGFP-FLAG-Ku70siR-WT

   Plasmid

   #46961

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   human Ku70 (XRCC6 Human)
   

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Promoter

   CMV Tet

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5 FRT TO myc Separase C2029A

   Plasmid

   #59822

   Purpose

   Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C2029A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLIX402 caPHF2

   Plasmid

   #91701

   Purpose

   inducible lentiviral expression of constitutively active PHF2

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   PHF2 (PHF2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   caPHF2; S757D, S899D, S954D, S1056D

   Promoter

   TRE promoter, Tet ON

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pPRIME-TREX-GFP-FF3

   Plasmid

   #11667

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a Tet-responsive promoter (TREX) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-GSX2-tTA-BleoR

   Plasmid

   #161748

   Purpose

   Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

   Depositor

   Elena Cattaneo

   Article

   Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

   Insert

   Advanced tTA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   endogenous GSX2 promoter

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57.1-MBD3L2

    Plasmid

    #106332

    Purpose

    All-in-one lentivector with tet-inducible MBD3L2

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    methyl-CpG binding domain protein 3 like 2 (MBD3L2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid

    #107421

    Purpose

    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    mutation of glutamate 77 to glutamine

    Promoter

    Tet

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIBA2-SpyTag-MBP

    Plasmid

    #107422

    Purpose

    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    Maltose-binding protein (MalE)
    

    Tags

    OmpA signal peptide, SpyTag, and StrepII

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5-FRT-TO-EGFP-RIP2_S176A

    Plasmid

    #131202

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

    Insert

    RIPK2 S176A (RIPK2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S176A

    Promoter

    pCMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA-sfGFP

    Plasmid

    #162689

    Purpose

    pFA vector control expressing sfGFP

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    sfGFP
    

    Tags

    C terminal 6x His tag

    Expression

    Bacterial

    Promoter

    PL tet promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRDA_103

    Plasmid

    #133460

    Purpose

    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKDsgRNA-pfkB

    Plasmid

    #102284

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    pfkB gRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFRT-TODestFLAGHA_RBPMS2_HindIII

    Plasmid

    #65666

    Purpose

    FLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII site

    Depositor

    Thomas Tuschl

    Article

    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    introduced HindIII site (final goal to exchange F…

    Promoter

    CMV, 2x Tet

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVBL0001

    Plasmid

    #242429

    Purpose

    Stable genomic integration of Opto-IRE1 (HsIRE1dLD-mCherry-CRY2clust) using the Flp-in system.

    Depositor

    Vladislav Belyy

    Article

    Smith et al bioRxiv [Preprint]. 2025 Jul 21:2025.07.

    Insert

    IRE1alpha (ERN1 Human)
    

    Tags

    mCherry, CRY2clust

    Expression

    Mammalian

    Mutation

    Deletion of the lumenal domain

    Promoter

    CMV, tet-inducible

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVUT-spC3GFP

    Plasmid

    #225487

    Purpose

    Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Secretable and permeable exoenzyme C3 transferase
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 30-251

    Promoter

    Human ubiquitin C

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

    Plasmid

    #205603

    Purpose

    Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

    Depositor

    Tarun Kapoor

    Article

    Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

    Insert

    γ-tubulinWT-RNAi-resistant-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G2228A, C2231T, C2237T, G2240A

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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