We narrowed to 4,182 results for: biorxiv

1. pLenti-rTetR(SE)-KRAB-2A-mTurquoise

   Plasmid

   #225690

   Purpose

   Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   rTetR-ZNF10 KRAB
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG and T2A-mTurquoise

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBK1302-AAV-EFSNC-dCjCas9-KRAB

   Plasmid

   #223147

   Purpose

   Expression of KRAB with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBK1312-AAV-EFSNC-dSaCas9-KRAB

   Plasmid

   #223157

   Purpose

   Expression of KRAB with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-MBP-TEVpCS-ADGRG7 CTF-FLAG

   Plasmid

   #217705

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGR7 (ADGRG7 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-415

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG

   Plasmid

   #217715

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRB2 (BAI2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-917

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

   Plasmid

   #217713

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRA3 (ADGRA3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-743

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 5HT2A-APEX2

   Plasmid

   #215558

   Purpose

   Stable, inducible expression of 5HT2A

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

   Plasmid

   #222990

   Purpose

   Bacterial expression of iGeoCas9(C1) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
   

   Use

   CRISPR

   Tags

   CL7, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA

   Plasmid

   #218870

   Purpose

   Mammalian expression of improved GABA sensor control (no change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2(no bind)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102G R168P

   Promoter

   CAG

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNeonGreen-C1-Nir1-631-894

    Plasmid

    #220089

    Purpose

    Truncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PA

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Use

    Tags

    NeonGreen-GGSGG

    Expression

    Mammalian

    Mutation

    amino acids 631-894

    Promoter

    CMV

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCL758

    Plasmid

    #219504

    Purpose

    Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNeonGreen-N1-Nir1-613-630

    Plasmid

    #220088

    Purpose

    N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Use

    Tags

    GGSGG-NeonGreen

    Expression

    Mammalian

    Mutation

    amino acids 613-630

    Promoter

    CMV

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-RSET-iGABASnFR2(no bind)-6xHis

    Plasmid

    #218887

    Purpose

    Bacterial expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2(no bind)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S99A F102G R168P

    Promoter

    T7

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid

    #216379

    Purpose

    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

    Depositor

    William Weis

    Article

    Bruguera et al bioRxiv 2024.02.03.578714

    Insert

    Frizzled-4 (Fzd4 Mouse)
    

    Use

    Baculovirus

    Tags

    6xHis and GP64 signal sequence

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

    Plasmid

    #216421

    Purpose

    Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    3xmyc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPbU6_hdhfr/yfcu

    Plasmid

    #216422

    Purpose

    Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

    Depositor

    Ellen Bushell

    Article

    Jonsdottir et al bioRxiv 2024.04.20.590404

    Type

    Empty backbone

    Use

    CRISPR; Expression in plasmodium berghei

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

    Plasmid

    #207171

    Purpose

    expression plasmid for Gal4DBD-HIF-CAD.myc expression

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    Gal4DBD-HIF1a-CAD Myc tag linker
    

    Use

    Tags

    2xMyc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-Duet_OPTN-thrombin-GST

    Plasmid

    #216843

    Purpose

    For expression in E. coli of OPTN (Internal ref. SMC1789)

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    Optineurin (OPTN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFastBac-Dual-GST-TEV-SINTBAD-mCherry

    Plasmid

    #208874

    Purpose

    For expression in insect cells of SINTBAD-mCherry

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MBP-TEVpCS-ADGRL4 CTFdeltaTA-FLAG

    Plasmid

    #217742

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL4 (ADGRL4 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-412

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge