We narrowed to 159,157 results for: addgene

1. pNM3724

   Plasmid

   #154112

   Purpose

   tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   tetO 7X::∆pes-10::GFP-C1::tbb-2 3'
   

   Use

   Rmce

   Tags

   Expression

   Worm

   Mutation

   Promoter

   not applicable

   Available since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

   Plasmid

   #174091

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV and T7

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5T5G (SETD8)

   Plasmid

   #101886

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SETD8 (KMT5A Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. FHUG+W (B2)

   Plasmid

   #74011

   Purpose

   lentiviral expression of EGFP and shRNA (from H1 promoter, empty)

   Depositor

   Oliver Schluter, Robert Malenka, Weifeng Xu

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiGuide-BC-start

   Plasmid

   #127168

   Purpose

   Entry plasmid for two-step guide-barcode cloning

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   N/A

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MXS_CMV

   Plasmid

   #62417

   Purpose

   MXS_chaining vector with CMV

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   CMV Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. ACE2 g1 + Cas9

   Plasmid

   #153011

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + ACE2 gRNA (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. MXS_P2A

   Plasmid

   #62433

   Purpose

   MXS_chaining vector with P2A

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   Self-cleaving peptide
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MXS_bGpA

   Plasmid

   #62424

   Purpose

   MXS_chaining vector with bGpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   beta-Globin poly A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. AKAP13

    Plasmid

    #73232

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AKAP13 (AKAP13 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 1976-2211

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only