We narrowed to 1,674 results for: mcherry expressing plasmid

1. Intron-Tagging-pX330-Cas9-Blast

   Plasmid

   #159741

   Purpose

   Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   donor plasmid targeting sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-63: ACTN2-mEGFP

   Plasmid

   #124607

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Insert

   ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

   Plasmid

   #37120

   Purpose

   DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   TdTomato-EGFP
   

   Use

   AAV and Cre/Lox; Cre-switch

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSL0341 (pTarget)

   Plasmid

   #200906

   Purpose

   Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   pTarget
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-GFAP-TurboID

   Plasmid

   #227179

   Purpose

   Co-expression of FLAG-tagged-TurboID and T2A-mCherry under the GFAP short promoter by AAV vector

   Depositor

   Junmin Peng

   Article

   Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

   Insert

   TurboID
   

   Use

   AAV

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP_short

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

   Plasmid

   #118274

   Purpose

   Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

   Depositor

   Dietmar Schmitz

   Article

   Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

   Inserts

   bPAC(WT)
   SthK
   

   Use

   AAV

   Tags

   His tag, P2A, mCherry, and myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CamK II

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CaMKII-TurboID

   Plasmid

   #227178

   Purpose

   Co-expression of FLAG-tagged TurboID and T2A-mCherry under the CaMKIIα short promoter by AAV vector

   Depositor

   Junmin Peng

   Article

   Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

   Insert

   TurboID
   

   Use

   AAV

   Tags

   FLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa_short

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-DIO-ChETA-TdTomato-WPRE-pA

   Plasmid

   #37755

   Purpose

   Cre-dependent expression of ChETA-tdTomato, for optogenetic excitation

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   ChETA-TdTomato
   

   Use

   AAV and Cre/Lox; Cre-on

   Tags

   TdTomato

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBLU-VIPR

   Plasmid

   #220498

   Purpose

   Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

   Depositor

   Alexander Espinosa

   Article

   Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-FAS-TdTomato-WPRE-pA

    Plasmid

    #37092

    Purpose

    Expresses tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites)

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    TdTomato
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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