We narrowed to 9,597 results for: EPO

1. AAVS1-Puro-CAG-GCaMP6s

   Plasmid

   #120896

   Purpose

   Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

   Depositor

   Xiaojun Lian

   Article

   Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

   Insert

   GCaMP6s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.2-DEST-MatryoshCaMP6s

   Plasmid

   #100025

   Purpose

   Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Use

   Tags

   V5 Epitope Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTACR TH1-p2aGFP

   Plasmid

   #135815

   Purpose

   CRISPR based on px330 p2aGFP targeting TH gene near stop codon

   Depositor

   Tim Ahfeldt, Lee Rubin

   Article

   Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

   Insert

   TH 1 guide
   

   Use

   CRISPR

   Tags

   p2a Myr-eGFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTACR TH2-p2aGFP

   Plasmid

   #135816

   Purpose

   CRISPR based on px330 p2aGFP targeting TH gene near stop codon

   Depositor

   Tim Ahfeldt, Lee Rubin

   Article

   Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

   Insert

   TH 2 guide
   

   Use

   CRISPR

   Tags

   p2a Myr-eGFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF-ABEmax

   Plasmid

   #164415

   Purpose

   EF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   ABEmax
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CaTCH BC1

   Plasmid

   #154195

   Purpose

   Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Insert

   CaTCH BC1
   

   Use

   Lentiviral; Catch barcode labeling

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CaTCH_empty-backbone

   Plasmid

   #157746

   Purpose

   Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

   Depositor

   Anna Obenauf

   Article

   Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

   Type

   Empty backbone

   Use

   Lentiviral; Catch barcode cloning

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CAG-DDdCas9VP192-T2A-EGFP-ires-puro

   Plasmid

   #69534

   Purpose

   DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   DD-dCas9VP192-T2A-EGFP
   

   Use

   Tags

   C-term T2A-EGFP and DHFR Destabilised Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-SpCas9-2A-GFP-noITR

   Plasmid

   #118415

   Purpose

   Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. TetO-FUW-VdC9BV

    Plasmid

    #62195

    Purpose

    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes

    Depositor

    Kam Leong

    Article

    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7

    Insert

    VP64dCas9BFPVP64
    

    Use

    Lentiviral

    Tags

    Two VP64s tagged to dCas9 fused to BFP

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive) Cas9 (dCas9)

    Promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB-TRE3G-SOX17

    Plasmid

    #104541

    Purpose

    PiggyBac vector encoding doxycycline-inducible human SOX17

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    SRY-box 17 (SOX17 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-TIE-V5/HIS

    Plasmid

    #205612

    Purpose

    Expression of human TIE receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    TIE receptor tyrosine kinase (TIE1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YFP-Rpp29-C1

    Plasmid

    #134545

    Purpose

    Expresses YFP-Rpp29 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    Rpp29 (POP4 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330.Pten.b

    Plasmid

    #66588

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCVL.Active/Repressed TLR (Sce)

    Plasmid

    #45575

    Purpose

    Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TetO-FUW-DN-REST

    Plasmid

    #61533

    Purpose

    Expresses DN-REST in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    REST (REST Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P73, Dominant negative REST (contains the Zf DNA-…

    Promoter

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG668 lifeAct-pFAST

    Plasmid

    #172866

    Purpose

    Expresses LifeAct-pFAST in mammalian cells (actin labeling)

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    LifeAct-pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-Gata2

    Plasmid

    #60665

    Purpose

    PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Gata2 (Gata2 Rat)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX330.Pten.a

    Plasmid

    #66587

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pFBP-2_6.sensor

    Plasmid

    #162800

    Purpose

    Yeast expression of the RNA-based sensor for fructose-1,6-bisphosphate #2_6

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    2_6_RNA-device
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge