Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 32,930 results for: Eng

Showing: 4231 - 4260 of 32930 results

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pBiT3.1-N_GEMIN5:1-739

Plasmid

#211124

Purpose

Mammalian expression of human GEMIN5 fragment M1 to A739. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

Gemin5:M1-A739 (GEMIN5 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

R682Q natural variant (VAR_033807)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_LRRK2:2142-2527

Plasmid

#211112

Purpose

Mammalian expression of human LRRK2 fragment R2142 to E2527. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LRRK2:R2142-E2527 (LRRK2 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

M2397T natural variant (VAR_024965)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_EIF3B:186-685

Plasmid

#211205

Purpose

Mammalian expression of human EIF3B fragment V186 to T685. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF3B:V186-T685 (EIF3B Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DENND3:894-1198

Plasmid

#211203

Purpose

Mammalian expression of human DENND3 fragment V894 to E1198. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DENND3:V894-E1198 (DENND3 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_GEMIN5:1-739

Plasmid

#211165

Purpose

Mammalian expression of human GEMIN5 fragment M1 to A739. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

Gemin5:M1-A739 (GEMIN5 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

R682Q natural variant (VAR_033807)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_LRRK2:2142-2527

Plasmid

#211151

Purpose

Mammalian expression of human LRRK2 fragment R2142 to E2527. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

LRRK2:R2142-E2527 (LRRK2 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

M2397T natural variant ( VAR_024965)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_KIF21A:1337-1653

Plasmid

#211213

Purpose

Mammalian expression of human KIF21A fragment L1337 to A1653. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

KIF21A:L1337-A1653 (KIF21A Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFBD-BirA_EIF2A:1-427

Plasmid

#210938

Purpose

Insect expression of human EIF2A fragment M1 to T427. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF2A:M1-T427 (EIF2A Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 4, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_COPB2:1-300

Plasmid

#210917

Purpose

Insect expression of human COPB2 fragment M1 to G300. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COPB2:M1-G300 (COPB2 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_DCAF1:1038-1400

Plasmid

#210883

Purpose

Insect expression of human DCAF1 fragment E1038 to E1400. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBiT3.1-C_NWD2:944-1662

Plasmid

#211319

Purpose

Mammalian expression of human NWD2 fragment S944 to S1662. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

NWD2:S944-S1662 (KIAA1239 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_KIF21A:1337-1653

Plasmid

#211287

Purpose

Mammalian expression of human KIF21A fragment L1337 to A1653. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

KIF21A:L1337-A1653 (KIF21A Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_EIF3B:186-685

Plasmid

#211279

Purpose

Mammalian expression of human EIF3B fragment V186 to T685. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF3B:V186-T685 (EIF3B Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_DENND3:894-1198

Plasmid

#211277

Purpose

Mammalian expression of human DENND3 fragment V894 to E1198. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DENND3:V894-E1198 (DENND3 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_NWD2:944-1662

Plasmid

#211245

Purpose

Mammalian expression of human NWD2 fragment S944 to S1662. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

NWD2:S944-S1662 (KIAA1239 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_DCAF1:1038-1400

Plasmid

#211052

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_DCAF1:1038-1400

Plasmid

#211053

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-N_COPB2:1-597

Plasmid

#211054

Purpose

Mammalian expression of human COPB2 fragment M1 to A597. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COPB2:M1-A597 (COPB2 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-C_DCAF1:1038-1400

Plasmid

#210963

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLF1-N_DCAF1:1038-1400

Plasmid

#210962

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBiT3.1-C_COPB2:1-597

Plasmid

#211055

Purpose

Mammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COPB2:M1-A597 (COPB2 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-PL

Plasmid

#20783

Depositor

Bryan Cullen

Article

Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pRSCqlux

Plasmid

#236109

Purpose

LuxCDABE reporter vector for Ralstonia pseudosolanacearum filamentous.

Depositor

Dehong Zheng

Article

Huang et al Phytopathol Res (2025) 7, 78. https://do

Insert

luxCDABE

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-miR21

Plasmid

#20381

Depositor

Bryan Cullen

Article

Zeng et al RNA. 2003 Jan . 9(1):112-23.

Insert

miR21 (MIR21 Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-6.20

Plasmid

#41002

Depositor

James Inglese

Article

Cheng et al Nat Methods. 2012 Oct;9(10):937. doi: 10

Insert

GSG-P2A-RLuc

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Nov. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-6.22

Plasmid

#41087

Depositor

James Inglese

Article

Cheng et al Nat Methods. 2012 Oct;9(10):937. doi: 10

Insert

GSG-P2A-RLuc

Use

Luciferase

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 13, 2012

Availability

Academic Institutions and Nonprofits only
pCI-6.24

Plasmid

#41003

Depositor

James Inglese

Article

Cheng et al Nat Methods. 2012 Oct;9(10):937. doi: 10

Inserts

GSG-P2A-RLuc
4XCRE + Minimal promoter

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
PX400 Campylobacter lari CF89-12 Cas9

Plasmid

#68336

Purpose

Campylobacter lari CF89-12 Cas9

Depositor

Feng Zhang

Insert

ClCas9

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRLG3A

Plasmid

#36921

Depositor

Manuela Martins-Green

Article

Zheng et al Transgenic Res. 2008 Aug;17(4):741-7. Ep

Inserts

red fluorescent protein
eGFP

Expression

Mammalian

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pCMV GATA3

Plasmid

#83818

Purpose

Mammalian expression of mouse GATA3

Depositor

Douglas Engel

Insert

GATA3 (Gata3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

Showing: 4231 - 4260 of 32930 results

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