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POC1544

Plasmid

#226485

Purpose

pMOLC360_VL_I10 (Chloramphenicol R) containing the insert I10 to be assembled in POC1520 (pMOBK360_VR) using the site-selective methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1545

Plasmid

#226486

Purpose

pMOLC360_VR_I11 (Chloramphenicol R) containing the insert I11 to be assembled in POC1520 (pMOBK360_VR) using the site-selective methylation protection

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1426

Plasmid

#221573

Purpose

Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1427

Plasmid

#221574

Purpose

Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pNK883

Plasmid

#219725

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn104

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn104

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn104

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-1α-p37-mClover2

Plasmid

#225500

Purpose

Expression of mClover2-tagged p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral

Tags

mClover2

Expression

Mammalian

Mutation

no

Promoter

EF1A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5a-p37WT-FLAG

Plasmid

#225498

Purpose

Expression of FLAG-tagged wildtype p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5a-p37SHPmut_FLAG

Plasmid

#225499

Purpose

Expression of FLAG-tagged mutated p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

F215A/E218A/QKL220-222AAA

Promoter

CMV promoter

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_phN

Plasmid

#223858

Purpose

4G-cloning donor for insect promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_phC

Plasmid

#223859

Purpose

4G-cloning donor for insect promoter and terminator, C-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNK880

Plasmid

#219726

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn105

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn105

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn105

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pNK879

Plasmid

#219727

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn108

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn108

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn108

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK887

Plasmid

#219728

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn110

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn110

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn110

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK882

Plasmid

#219729

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn159x

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn159x

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn159x

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK881

Plasmid

#219730

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1556x

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn1556x

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn1556x

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK884

Plasmid

#219731

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1569x

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn1569x

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn1569x

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK6030

Plasmid

#219732

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1637x

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn1637x

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn1637x

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK885

Plasmid

#219733

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1824x

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn1824x

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn1824x

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK878

Plasmid

#219734

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn1904x

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn1904x

Use

Synthetic Biology

Expression

Plant

Promoter

MinSyn1904x

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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C1C2CMUNC2C

Plasmid

#218674

Purpose

Insect cell expression of a rat MUNC13-1 fragment containing domains C1C2BMUNC2C

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

MUNC13 (Unc13a Rat)

Tags

GST

Expression

Insect

Mutation

Amino acids1407-1453 deleted

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1_mNeonGreen_Ptet-2_mCherry

Plasmid

#213839

Purpose

Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen, mCherry

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pIVEX2.3-AC17-5c-frGFP

Plasmid

#217525

Purpose

AC17-5c riboswitch-regulated GFP reporter. The gene is inserted into downstream of T7 promoter.

Depositor

Keisuke Fukunaga

Article

Ishii et al Chem Commun (Camb). 2024 Jun 4;60(46):59

Insert

AC17-5c riboswitch-regulated folding reporter GFP

Use

In vitro transcription-translation

Promoter

T7

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD

Plasmid

#216396

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-356

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Expression

Worm

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS160

Plasmid

#215682

Purpose

Guide only plasmid targeting chrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Expression

Worm

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pIBA2-IntHA453-SpyTag

Plasmid

#198039

Purpose

Expression plasmid for producing SpyTagged intimin

Depositor

Jack Leo

Article

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Tags

SpyTag

Expression

Bacterial

Mutation

double HA tag inserted at position 453; lacks nat…

Promoter

tet

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mTB3-FLAG tag

Plasmid

#210204

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the FLAG-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

FLAG-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mTB3-AviD tag

Plasmid

#210203

Purpose

Type 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.

Depositor

Brian Kay

Article

Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

Insert

AviD-tag

Expression

Bacterial

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge